Optimiranje metode ekstrakcije polifenola iz brokule (Brassica oleracea var. botrytis L.) mikrovalnim zračenjem

undergraduate thesis

• Title (english): Optimized microwave-assisted extraction of total phenolics from broccoli (BRASSICA OLERACEA VAR. BOTRYTIS L.) and its antioxidant activity
• Author: Nikola Zima
• Mentor: Ivana Radojčić Redovniković (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Cell Technology, Application and Biotransformations); Zagreb
• Defense date and country: 2011-07-22, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Cilj ovoga rada bio je ispitati utjecaj procesnih parametara ekstrakcije potpomognute mikrovalnim zračenjem (temperatura, vrijeme, otapalo i snaga) na sadržaj polifenolnih tvari i antioksidacijski kapacitet ekstrakata brokule te odrediti optimalne uvjeta navedenih procesnih parametara metodom odzivnih površina. Određeni su optimalni uvjeti ekstrakcije polifenolnih spojeva iz brokule: temperatura 71,73 °C, snaga 160,76 w, volumni udio metanola u vodenoj otopini metanola 71,73 %, v/v te vrijeme 16,55 minuta. Pri navedenim uvjetima izračunato je da bi se mogao ostvariti maksimalni prinos ukupnih polifenola iz brokule od 21,44 mgGAE gs.t.-1 te antioksidacijski kapacitet ekstrakta brokule od 177,107 µmol trolox ekvivalenta g-1 s.t.

Abstract (english)

The effects of temperature (50 90 °C), solvent composition (50 90%, methanol/water, v/v ), extraction time (30 90 minute) and microwave power (100-200 W) such as their interaction on extractability of total phenolic content and antioxidant capacity of broccoli extracts were studied. The optimal conditions for the phenolic extraction were determined by response surface methodology (71.73 °C, 160.76 W, 71.73%, v/v methanol/water and 16.55 min). At this condition, the yield of total phenolic content was predicted to be 21.44 mgGAE g dw.-1, and antioxidant capacity of broccoli extracts of 177.107 μmol Trolox equivalents gdw -1.

• Keywords: polifenoli; antioksidacijski kapacitet; ORAC; brokula; mikrovalno zračenje; metodom odzivnih površina
• Keywords (english): broccoli; ORAC; polyphenols; microwave-assisted extraction; response surface methodology
• Language: croatian
• URN:NBN: urn:nbn:hr:159:496085
• Study programme: Title: Biotechnology; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-05-19 09:24:13