Inhibicija rasta patogenih mikroorganizama pomoću cvijeta bazge

master's thesis

• Title (english): Inhibition of pathogenic microorganisms growth by elder flowers
• Author: Petar Gaić
• Mentor: Ksenija Markov (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for General Microbiology and Food Microbiology); Zagreb
• Defense date and country: 2011-05, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Food Technology

Abstract

Biljke i biljni proizvodi mogu predstavljati izvor prirodnih alternativa za poboljšanje sigurnosti hrane. Cilj ovog rada bio je ispitati količinu ukupnih fenola, flavonoida i antimikrobnu aktivnost ekstrakata cvjetova samonikle bazge (Sambucus nigra L.) ubranih na različitim geografskim područjima Republike Hrvatske (područje grada Zagreba, grada Pule, Like i kultivirana bazga), prema odabranim test mikroorganizmima: Echerichia coli ZMPBF 3041, Staphylococcus aureus ZMPBF 3048, Salmonella spp. ZMPBF 3046 i Listeria monocytogenes ATCC 2256. Ukupni fenoli određeni su na temelju kolorne reakcije koja je posljedica reakcije fenola s Folin–Ciocalteu-ovim reagensom te je primjenom spektrofotometrijske metode mjeren intenzitet nastalog obojenja pri 760 nm. Flavonoidi su određeni na temelju svojstva da s aluminijevim kloridom (AlCl3) (1:10) tvore kompleks, a intenzitet obojenja je proporcionalan količini prisutnih flavonoida. Za određivanje antimikrobnog učinka i stupnja inhibicije pojedinih ekstrakta bazge je primjenjena turbidimetrijska metoda. Rezultati pokazuju da svi ekstrakti samonikle bazge sadrže veću koncentraciju ukupnih fenola od ekstrakta kultivirane bazge, dok je najveća koncentracija flavonoida u ekstraktu samonikle bazge iz područja Like. Svaki ekstrakt pokazuje i dobro inhibitorno djelovanje, no najbolju antimikrobnu aktivnost posjeduje ekstrakt samonikle bazge ubran na području Pule.

Abstract (english)

Plants and plant products may represent a source of natural alternatives for improving food safety. The aim of this study was to examine the amount of total phenols, flavonoids and the antimicrobial activity of extracts of wild elder flowers (Sambucus nigra L.) collected from different geographic regions of Croatian (Zagreb, Pula, Lika and cultivated elderberry), against selected bacteria: Echerichia coli ZMPBF 3041, Staphylococcus aureus ZMPBF 3048, Salmonella spp. ZMPBF 3046 i Listeria monocytogenes ATCC 2256. Total phenols were determined on the basis of color vision feedback, which is the result of reaction of phenol with Folin-Ciocalteu's reagent and was measured using the spectrophotometric method of staining intensity generated at 760 nm. Flavonoids are determined based on the properties that they form a complex with aluminum chloride (AlCl3) (1:10), and the resulting color is proportional to the amount of flavonoids present. Turbidimetric method was applied to determine the antimicrobial effect and the degree of inhibition of the elderberry extract. The results showed that all extracts of wild elderberries contain a higher concentration of total phenols than the extract of cultured elder, while the highest concentration of flavonoids is in the extract of wild elderberries from Lika. Each extract shows good inhibitory activity, but the best antimicrobial activity was shown by wild elderberry extract from Pula.

• Keywords: Sambucus nigra L.; ukupni fenoli; flavonoidi; antimikrobna aktivnost
• Keywords (english): Sambucus nigra L.; total phenols; flavonoids; antimicrobial activity
• Language: croatian
• URN:NBN: urn:nbn:hr:159:552948
• Study programme: Title: Biotechnology; specializations in: Biochemical Engineering; Course: Biochemical Engineering; Study programme type: university; Study level: graduate; Academic / professional title: diplomirani inženjer biotehnologije; smjer biokemijsko inženjerstvo (diplomirani inženjer biotehnologije; smjer biokemijsko inženjerstvo)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-05-30 11:27:29