Utjecaj Sec14p proteina i aspartatnih proteaza iz porodice japsina na ugradnju Scw4p u staničnu stijenku kvasca Saccharomyces cerevisiae

master's thesis

• Title (english): Effect of Sec14p protein and yaspin family aspartic proteases on the attachment of Scw4p protein to the yeast Saccharomyces cerevisiae cell wall
• Author: Ana Rušev
• Mentor: Renata Teparić (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Chemistry and Biochemistry); (Laboratory for Biochemistry); Zagreb
• Defense date and country: 2011-09-27, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Protein Scw4p je u staničnu stijenku kvasca vezan na dva načina. Poznato je da je različit način vezanja posljedica procesiranja ovoga proteina tijekom kojeg se odcjepljuje 11 aminokiselina na N-terminalnom dijelu proteina. Procesirani protein se u stijenku veže nekovalentno dok se neprocesirana frakcija Scw4p veže u stijenku kovalentnim vezama. U procesiranju Scw4p sudjeluje Kex2p proteaza, no nije poznato da li posredno ili neposredno. U ovom radu je pomoću soja kvasca 5ypsΔ sa disruptiranim genima za svih pet japsina dokazana uloga japsinskih proteaza u procesiranju Scw4p. Uzgojem stanica u uvjetima koji izazivaju stanični stres, a za koje je poznato da utječu na indukciju i aktivnost Kex2p i pojedinih japsina je potvrđena uloga ovih proteaza u procesiranju i posljedično načinu ugradnje Scw4p u stijenku. Eksperimentalno je istražen i način transporta dviju formi Scw4p, koristeći termosenzitivni mutant u SEC14 genu.

Abstract (english)

Scw4p is a yeast cell wall protein that is attached to the wall in two different ways. Cause of this difference in attachment manner is the proteolitic processing of the protein, when 11 amino acid residues are split off from the N-terminal side of the protein. Processed Scw4p proteins are non-covalently attached to the cell wall while the unprocessed fraction is attached by a covalent linkage. Kex2p protease is involved in the processing reaction but it is not yet known whether directly or indirectly. This research demonstrates the effect of yapsin proteases to this phenomenon by using yeast strains 5ypsΔ with disrupted genes which encode for all five yapsins. By growing cells under conditions that induce cellular stress, which is known to affect the induction and activity of Kex2p and individual yapsins, the role of these proteases in processing and consequentially attachment was confirmed . Transport of Scw4p protein is investigated using temperature-sensitive mutant strain sec14.

• Keywords: Scw4p; Kex2p; japsini; SEC14
• Keywords (english): Scw4p; Kex2p; yapsins; sec14
• Language: croatian
• URN:NBN: urn:nbn:hr:159:583938
• Study programme: Title: Molecular Biotechnology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-05-31 08:48:22