Enzimski katalizirana hidroliza (R, S)-1-feniletil acetata u eutektičkim otapalima

undergraduate thesis

• Title (english): Enzymic catalytic hydrolysis (R, S)-1-phenylethyl acetate in deep eutectic solvents
• Author: Martina Rajn
• Mentor: Marina Cvjetko Bubalo (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Cell Technology, Application and Biotransformations); Zagreb
• Defense date and country: 2017-07-17, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Visokoučinkoviti i ekološki prihvatljivi procesi proizvodnje industrijski važnih kiralnih građevnih blokova, biokatalitičkih postupaka te postupaka u kojima se primjenjuju zelena otapala, posljednjih su godina predmet istraživanja. Cilj ovog rada bio je istražiti dvije vrste biokatalizatora, izoliranu lipazu CALB i korijen mrkve, za hidrolizu (R, S)-1-feniletil acetata u različitim eutektičkim otapalima. Obzirom na praćeno iskorištenje reakcije, enantiomerni višak, specifičnu produktivnost enzima i volumetrijsku produktivnost enzima može se zaključiti da su prirodna eutektička otapala superiornija za hidrolizu (R, S)-1-feniletil acetata s izoliranom lipazom od hlapljivih organskih otapala. Hidrolizom pomoću mrkve nema značajnijih pomaka u iskorištenju i produktivnosti u odnosu na organska otapala, no vidljivo je poboljšanje u enantioselektivnosti. Rezultati prikazani u ovom istraživanju doprinos su razvoju učinkovitih, ekonomičnih i održivih procesa za hidrolizu industrijski važnih spojeva.

Abstract (english)

Highly efficient and environmentally-acceptable processes for the production of industrially important chiral building blocks, biocatalytic processes and green solvent-based processes have been the subject of research for the last few years. The aim of this study was the application of two biocatalysts, isolated lipase CALB and carrot root, for hydrolysis (R, S)-1-phenylethyl acetate within natural deep eutectic solvents. Based on the observed reaction yields, enantiomeric excess, specific enzymatic productivity and enzyme volumetric productivity, it can be concluded that natural deep eutectic solvents were superior to hydrolysis of (R, S)-1-phenylethyl acetate with isolated lipase according to volatile organic solvents. Hydrolysis by carrot had no significant impact on yield and productivity compared to organic solvents, but there was a clear improvement in enantioselectivity. The presented results contribute to the development of an efficient, economical and sustainable processes for the hydrolysis of industrially important compounds.

• Keywords: biotransformacije; lipaza; mrkva; prirodna eutektička otapala; (R; S)-1-feniletil acetat
• Keywords (english): biotransformations; carrot root; lipase; natural deep eutectic solvents; (R; S)-1-phenylethyl acetate
• Language: croatian
• URN:NBN: urn:nbn:hr:159:381555
• Study programme: Title: Biotechnology; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2017-07-20 10:55:57