Određivanje benzojeve i sorbinske kiseline tekućinskom kromatografijom visoke djelotvornosti i njena primjena na uzorcima hrane

master's thesis

• Title (english): Determination of benzoic and sorbic acid by high performance liquid chromatography and its application on food samples
• Author: Renata Mitton
• Mentor: Jasna Vorkapić-Furač (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Cell Technology, Application and Biotransformations); Zagreb
• Defense date and country: 2010-05-17, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Benzojeva i sorbinska kiselina su konzervansi koji se često koriste pri konzerviranju različitih vrsta prehrambenih proizvoda. Konzervansi se dodaju radi stabiliziranja proizvoda, produljenja njegove održivosti i sprječavanja kvarenja odnosno mikrobiološke kontaminacije. Na taj način se produžuje trajnost namirnice i omogućuje transport lako pokvarljive robe na veće udaljenosti. Cilj ovog rada bio je utvrditi količine benzojeve i sorbinske kiseline koje sadrže 30 uzoraka prehrambenih proizvoda koja se trenutno nalaze na hrvatskom tržištu. Određivanje benzojeve i sorbinske kiseline provedeno je tekućinskom kromatografijom obrnutih faza i utvrdilo se je su li dobivene količine u skladu sa Pravilnikom o prehrambenim aditivima. Istraživanje je pokazalo da većina uzoraka sadrže znatno niže količine konzervanasa od maksimalno dozvoljenih količina navedenih u Pravilniku. Međutim, dva uzorka koji prelaze preporučenu razinu konzervanasa ukazuju na potrebu za strožom regulacijom uporabe konzervanasa u prehrambenoj industriji, na potrebu obaveznog postupka analize hrane te da količina dodanih aditiva u namirnicama treba biti strogo kontrolirana .

Abstract (english)

Benzoic and sorbic acid are commonly used preservatives, applied to a wide variety of foods. Preservatives are added to stabilize products, extend sustainability, prevent spoilage or microbial contamination, prolong shelf-life and enable transportation of easily perishable goods to major destinations. The objective of this research was to determine the quantities of benzoic and sorbic acid in 30 foodstuff samples present at the Croatian market. The reversed phase liquid chromatography (RPLC) was used to determine whether the results are in accordance with the Regulation of Food Additives. The research has shown that most samples contain significantly lower concentrations than the maximum permitted levels. However, two samples which exceed the recommended levels of preservatives indicate the necessity of a more rigorous regulation of applied preservatives in food industry, exigency of a mandatory step of food analysis and severe control of food additives.

• Keywords: benzojeva kiselina; konzervansi; sorbinska kiselina; tekućinska kromatografija visoke djelotvornosti
• Keywords (english): benzoic acid; high performance liquid chromatography; preservatives; sorbic acid
• Language: croatian
• URN:NBN: urn:nbn:hr:159:571594
• Study programme: Title: Biotechnology; specializations in: Biochemical Engineering; Course: Biochemical Engineering; Study programme type: university; Study level: graduate; Academic / professional title: diplomirani inženjer biotehnologije; smjer biokemijsko inženjerstvo (diplomirani inženjer biotehnologije; smjer biokemijsko inženjerstvo)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-08-29 08:42:12