Anotacija genskih nakupina poliketida i hibridnih sustava u genomu bakterije Streptomyces scabies

master's thesis

• Title (english): Anotaion of polyketide and hybrid bgene-clusters in Streptomyces scabies genome
• Author: Ida Trninić
• Mentor: Daslav Hranueli (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Section for Bioinformatics); Zagreb
• Defense date and country: 2009-11-10, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Poliketidi i neribosomalno sintetizirani peptidi su najznačajniji predstavnici sekundarnih metabolita. U zadnje vrijeme sve veću pozornost privlače i hibridni sustavi. Mehanizmi sinteze navedenih sekundarnih metabolita zasnivaju se na enzimskim kompleksima nazvanim poliketid sintaze (PKS) i sintetaze neribosomalnih peptida (NRPS). U biosintezi hibridnih sustava uključeni su enzimski kompleksi obje skupine. Geni čiji enzimi sudjeluju u biosintezi sekundarnih metabolita obično su organizirani u genske nakupine. Cilj ovog diplomskog rada bio je sistematizirati znanje o dosada istraženim i detaljno opisanim biosintetskim mehanizmima sustava PKS i hibrida. Takav pristup trebao bi dovesti do bolje klasifikacije navedenih sekundarnih metabolita. Taj je pristup testiran na novosekvencioniranom i do sada neopisanom genomu bakterije Streptomyces scabies. Osnovni alat koji je upotrijebljen u ovom radu jest bioinformatički programski paket ClustScan razvijen na Prehrambeno-biotehnološkom fakultetu Sveuĉilišta u Zagrebu.

Abstract (english)

Polyketides and nonribosomal peptides represent most valued bioactive compounds among secondary metabolites. Recently their hybrid systems also attract a lot of attention. The biosynthetic mechanisms involved in the production of these compounds are based on large multifunctional enzymes called polyketide synthases (PKS) and nonribosomal peptide synthetases (NRPS). Genes coding for these systems in bacteria or fungi are usually arranged in the gene clusters. The goal of this work was to try to systematize existing knowledge on these enzymes and produce a better, more detailed classification which could help distinguish them on the level of DNA sequence. This classification was tested on a newly sequenced un-annotated genome of Streptomyces scabies. The main bioinformatic tool used in this work has been the ClustScan program package, a computer program developed at the Faculty of Food Technology and Biotechnology.

• Keywords: PKS genske nakupine; hibridne genske nakupine; genom bakterije Streptomyces scabies; raĉunalni programski paket ClustScan
• Keywords (english): PKS gene-clusters; hybrid gene-clusters; Streptomyces scabies genome; ClustScan program package
• Language: croatian
• URN:NBN: urn:nbn:hr:159:985941
• Study programme: Title: Biotechnology; specializations in: Biochemical-Microbiology; Course: Biochemical-Microbiology; Study programme type: university; Study level: graduate; Academic / professional title: diplomirani inženjer biotehnologije; smjer biokemijsko-mikrobiološki (diplomirani inženjer biotehnologije; smjer biokemijsko-mikrobiološki)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-09-01 07:55:45