Biodostupnost i antioksidacijski potencijal bioaktivnih komponenti iz pokožice grožđa u C57BL/6 miša

master's thesis

• Title (english): Bioavailability and antioxidative potential of bioactive compounds of grape skin from C57BL/6 mouse
• Author: Monika Fumić
• Mentor: Irena Landeka Jurčević (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Food Quality Control); (Laboratory for Food Chemistry and Biochemistry); Zagreb
• Defense date and country: 2016-09-27, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Nutrition

Abstract

Biodostupnost bioaktivnih spojeva, prvenstveno antocijana, iz pokožice grožđa istražena je na modelu laboratorijskih životinja (C57BL/6 miš) kroz 30 dana tretmana. Eksperimentalne grupe su činile 2 grupe životinja (težine 30±2,51 g): grupa I- kontrolna grupa (0,2 mL fiziološke otopine) te grupa II- tretirana ekstraktom pokožice grožđa (GSE) (100 mg/kg∕dan). Istraživani su i biomarkeri oksidacijskog stresa u homogenatu tkiva bubrega i jetre, malonildialdehid (MDA), karbonilirani proteini (PC), reducirani glutation (GSH), supeoksid dismutaza (SOD) i katalaza (CAT). Rezultati pokazuju da je tretman s GSE statistički značajno (ANOVA, p<0,05) smanjio aktivnosti MDA i PC u bubregu i jetri miša u odnosu na kontrolnu skupinu životinja što se može pripisati polifenolima prisutnim u ekstraktu pokožice grožda, dok su ostale grupe ostale na razini kontrolne skupine. Koncentracije GSH, SOD i CAT u bubregu statistički su značajno (ANOVA, p<0,05) povećane u svim grupama miševa u odnosu na kontrolnu grupu, što ukazuje na antioksidacijski učinak pokožice grožđa na smanjenje oksidacijskog stresa.

Abstract (english)

Bioavailability of bioactive compounds, primarily anthocyanins, from grape skin extract has been investigated in laboratory animals (C57BL/6 mouse). The animals were treated for 30 days and divided into 2 experimental groups (weight 30±2,51 g): group I - control group (0,2 mL saline solution) and group II- treated with grape skin extract (100 mg/kg∕day). Biomarkers of oxidative stress which were studied in kidney and liver tissue homogenates were malondialdehyde (MDA), carbonylated proteins (PC), reduced glutathione (GSH), superoxide dismutase (SOD) and catalase (CAT). The results show that GSE statistically significant (ANOVA, p <0.05) reduced the activity of MDA and PC in the kidney and liver of a mouse which was compared with control group of animals. That effect can be attributed to the polyphenols present in the grape skin extract. The other groups were unchanged and close to the control group. The concentrations of GSH, SOD and CAT were statistically significant (ANOVA, p <0.05) and increased in all groups of mice compared to the control group, which indicates the antioxidant effect of GSE and potential of reducing oxidative stress.

• Keywords: biodostupnost; antioksidacijski potencijal; ekstakt pokožice grožđa (Vitis Vinifera)
• Keywords (english): bioavailability; antioxidant potential; grape skin extract (Vitis Vinifera)
• Language: croatian
• URN:NBN: urn:nbn:hr:159:395302
• Study programme: Title: Nutrition; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra nutricionizma (magistar/magistra nutricionizma)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2017-04-25 07:14:36