Priprava i karakterizacija prirodnih eutektičnih otapala

master's thesis

• Title (english): Preparation and characterization of natural deep eutectic solvent
• Author: Marina Milešević
• Mentor: Ivana Radojčić Redovniković (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Cell Technology, Application and Biotransformations); Zagreb
• Defense date and country: 2016-06-27, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Prirodna eutektična otapala (eng. Natural Deep Eutectic Solvents, NADES) su nova generacija ekološki prihvatljivih otapala sa mogućim primjenama u različitim industrijskim područjima. Definiraju se kao smjesa dviju ili više komponenata koje se međusobno mogu povezati vodikovim vezama te koje u određenom omjeru imaju niže talište nego pojedinačne komponente smjese. Zbog mogućnosti tvorbe NADES iz širokog spektra spojeva moguće je prilagoditi njihova svojstva određenoj primjeni. U ovom radu pripravljeno je osam različitih NADES u različitim molarnim odnosima (kolin klorid:jabučna kiselina, prolin:jabučna kiselina, kolin klorid:prolin:jabučna kiselina, betain:jabučna kiselina, jabučna kiselina:glukoza, jabučna kiselina:glukoza:glicerol, kolin klorid:limunska kiselina, betain:limunska kiselina). Fizikalno-kemijska svojstva kao što su gustoća, viskoznost, indeks loma, električna vodljivost i pH su izmjerena i analizirana kao funkcija različitih udjela vode u otapalima (10, 30 i 50 % v/v). Također, provedena je i biološka karakterizacija ispitivanjem njihove in vitro citotoksičnosti na humanim stanicama HeLa i HEK293T te je izmjeren njihov antioksidacijski kapacitet ORAC metodom. Budući da pripravljeni NADES ne pokazuju citotoksični učinak na tretirane stanice, imaju antioksidativna svojstva, te se fizikalno-kemijska svojstva mogu pozitivno prilagoditi povećanjem udjela vode postoji mogućnost njihove primjene u raznim tehnološkim procesima.

Abstract (english)

Natural deep eutectic solvents (NADES) are new generation of environmentally friendly solvents with possible applications in various industrial fields. They are defined as a mixture of two or more components which can be connected to each other by hydrogen bonds, and which in a certain ratio have lower melting points than the individual components of the mixture. Because of the possibility of forming NADES from a wide range of compounds it is possible to adjust their properties to particular application. In this study, eight different NADES was prepared at different molar ratios (choline chloride: malic acid, proline: malic acid, choline chloride: proline: malic acid, betaine: malic acid, malic acid: glucose, malic acid: glucose: glycerol, choline chloride: citric acid, betaine: citric acid). Physico-chemical properties such as density, viscosity, refractive index, electrical conductivity and pH were measured and analyzed as a function of water content in the solvent (10, 30 and 50% v/v). Also, a biological characterization was conducted by exemination of their in vitro cytotoxicity on human cells HeLa and HEK293T and measurement of their antioxidant capacity with ORAC method. Because prepared NADES doesn't show cytotoxic effects toward treated cells, has antioxidant properties and the physico-chemical properties can be positively adjusted by increasing the water content, there is a possibility of their application in various technological processes.

• Keywords: prirodna eutektična otapala; udio vode; fizikalno-kemijska svojstva; citotoksičnost; antioksidacijski kapacitet
• Keywords (english): natural deep eutectic solvents; water content; physico-chemical properties; cytotoxicity; antioxidative capacity
• Language: croatian
• URN:NBN: urn:nbn:hr:159:595652
• Study programme: Title: Molecular Biotechnology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2017-04-25 08:06:26