| Abstract | Za određivanje učinka vitamina E pri citotoksičkom učinku herbicida atrazina, korištena je stanična linija ovarija kineskog hrčka (CHO-K1). CHO-K1 stanice rasle su u monosloju na 37˚C u atmosferi 95% zraka i 5% CO2, u mediju za uzgoj Dulbecco's MEM uz dodatak 5% NCS-a (Newborn Calf Serum). Metodom Trypan Blue praćen je citotoksički učinak atrazina u koncentraciji 20, 40, 60 i 80 μg/mL medija za uzgoj i učinak vitamina E u koncentraciji 25 i 50 μg/mL medija za uzgoj pri citotoksičkom učinku atrazina. Citotoksički učinak praćen je tijekom 72 sata pri početnoj koncentraciji stanica 2x104 stanica/mL. Nakon 72 sata učinak vitamina E na dinamiku rasta stanica u koncentraciji 25 μg/mL medija za uzgoj iznosi 117 %, a vitamina E u koncentraciji 50 μg/mL medija za uzgoj iznosi 124,5% u odnosu na kontrolnu vrijednost. Pri koncentraciji atrazina od 20 μg/mL medija za uzgoj inhibicija rasta iznosi 14,29%, pri 40 μg/mL medija za uzgoj 25,82% pri 60 μg/mL medija za uzgoj 38,71% dok pri koncentraciji atrazina od 80 μg/mL medija za uzgoj iznosi 48,29%. Pri istim koncentracijama atrazina uz dodatak vitamina E u koncentraciji 25 μg/mL medija za uzgoj inhibicija rasta iznosi 11,29%, 35,96%, 36,29% i 42,52%. Uz dodatak vitamina E u koncentraciji 50 μg/mL medija za uzgoj pri koncentraciji atrazina od 20 μg/mL medija za uzgoj inhibicija atrazinom nije prisutna, dok kod slijedećih korištenih koncentracija atrazina inhibicija rasta iznosi 18,32%, 29,49% i 28,77%. Vitamin E pokazao je protektivni mehanizam i uz njegovu prisutnost CHO stanice su bolje rasle. |
| Abstract (english) | In order to determine influence of vitamin E during the cytotoxic effect of herbicide atrazine, Chinese Hamster Ovary (CHO-K1) cell line was used. CHO-K1 cells were cultivated at 37˚C in the atmosphere of 95% air and 5% CO2, in the medium Dulbecoo´s MEM with 5% NCS (Newborn Calf Serum). Cytotoxic effect of atrazine in concentrations of 20, 40, 60 and 80 μg/mL of cultivating medium and effect of vitamin E in concentratcions of 25 and 50 μg/mL of cultivating medium during the cytotoxic influence of atrazine were determined by Trypan Blue colorimetric method. After 72 hours, effect of vitamin E on cell growth dynamics in concentration 25 μg/mL of cultivating medium was 117%, and vitamin E in concentration 50 μg/mL of cultivating medium was 124,5%, compared to control value. At atrazine concentration of 20 μg/mL of cultivating medium growth inhibition was 14,29% at concentration of 40 μg/mL of cultivating medium growth inhibition was 25,82% at 60 μg/mL of cultivating medium, growth inhibition was 38,71%, and at atrazine concentration of 80 μg/mL of cultivating medium growth inhibition was 48,29%. At the same atrazine concentrations, with the addition of vitamin E at concentration 25 μg/mL of cultivating medium, growth inhibition was 11,29%, 35,96%, 36,29% and 42,52%. With the addition of vitamin E at concentration 50 μg/mL of cultivating medium at atrazine concentration of 20 μg/mL of cultivating medium there was no growth inhibition and at atrazine concentration of 40, 60 and 80 μg/mL of cultivating medium growth inhibition was 18,32%, 29,49% and 28,77%, respectivly. Vitamin E has shown protective mechanism and positive effect on CHO cells proliferation. |
