Spektrofotometrijsko određivanje polifenola u etanolnim ekstraktima ružmarina (Rosmarinus officinalis)

undergraduate thesis

• Title (english): Spectrophotometric determination of polyphenols from ethanolic extracts of rosemary (Rosmarinus officinalis)
• Author: Leonina Moslavac
• Mentor: Antonela Ninčević Grassino (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Chemistry and Biochemistry); (Laboratory for Analytical Chemistry); Zagreb
• Defense date and country: 2017-09-08, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Mali broj znanstvenih radova je pokazao da polifenoli kao aktivne komponente biljnog materijala, ključne za održavanje ravnoteže ljudskog organizma, osim antioksidacijskih posjeduju i antikorozijske osobine. Zbog izražene sposobnosti adsorpcije na metale i metalne legure, zauzimaju značajno mjesto pri ispitivanju antikorozijskog djelovanja. U ovom radu pripravljeni su etanolni (30 i 70 %, v/v) ekstrakti osušenih i usitnjenih listova ružmarina, primjenom ultrazvuka visokog intenziteta i refluksiranja. Procesni parametri primjenjeni kod estrakcije ultrazvukom bili su vrijeme od 3, 6 i 9 min i amplituda od 60, 80 i 100 % te vrijeme ekstrakcije od 1,5 i 3 h kod metode refluksiranja. Maseni udio ukupnih fenola i flavonoida u pripravljenim ekstraktima određen je UV/Vis spektrofotometrijskim testom. Ekstrakti s najvećim udjelima polifenola, dobiveni upotrebom 70 %-tnog etanola, pri vremenu ekstrakcije od 9 min i 100 %-tnoj amplitudi (optimalni parametri) koristit će se u njihovim daljnjim ispitivanjima kao antikorozijskih supstancija.

Abstract (english)

A few scientific papers have shown that polyphenols as active components of plant materials relevant for maintaining balance in human organism, besides antioxidants, also possess anticorrosive properties. Due to its capability of adsorption on metals and metal alloys, they occupied important place in investigations of anticorrosion activity. In this paper, ethanolic (30 and 70 %, v/v) extracts of dried and milled rosemary leaves are prepared by application of high intensity ultrasound and refluxing methods. Process parameters applied for ultrasound extraction were time of 3, 6, 9 min and amplitude of 60, 80 and 100 %, and extraction time of 1,5 and 3 h for reflux method. Mass fractions of total phenols and flavonoides are determined by UV/Vis spectrophotometric test. Extracts with the highest mass fractions of polyphenols, obtained by 70 % ethanol at extraction time of 9 min and 100 % amplitude (optimal conditions) will be used for theirs further examination as anticorrosive substance.

• Keywords: antikorozijska i antioksidacijska svojstva; polifenoli; ružmarin; ultrazvuk; UV/Vis spektrofotometrija
• Keywords (english): anticorrosion and antioxidant activity; polyphenols; rosemary; ultrasound; UV/Vis spectrophotometry
• Language: croatian
• URN:NBN: urn:nbn:hr:159:555137
• Study programme: Title: Biotechnology; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2017-09-13 12:55:54