Učinak mravlje i levulinske kiseline na rast i akumulaciju lipida u plijesni Mortierella isabellina

undergraduate thesis

• Title (english): Effect of formic and levulinic acid on growth and lipid accumulation of fungus Mortierella isabellina
• Author: Ines Peremin
• Mentor: Mirela Ivančić - Šantek (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology); Zagreb
• Defense date and country: 2017-09-18, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Plijesan Mortierella isabellina potencijalni je kandidat za proizvodnju lipida na lignoceluloznim hidrolizatima. Predobradom lignocelulozne sirovine nastaju brojni nusproizvodi koji značajno smanjuju aktivnost celulaza, mikrobni rast i akumulaciju proizvoda. Istražen je učinak pojedinačnih alifatskih kiselina na rast i akumulaciju lipida tijekom submerznog uzgoja plijesni, pri čemu je kao izvor ugljika korištena glukoza. Rezultati su pokazali da je inhibicijski učinak mravlje kiseline jači u odnosu na levulinsku kiselinu. Značajan pad koncentracije biomase i lipida uočen je pri koncentracijama levulinske kiseline iznad 1,111 g L-1. Utvrđeno je da mravlja kiselina pri nižim koncentracijama pospješuje rast biomase plijesni i biosintezu lipida. Pri koncentracijama levulinske kiseline većim od 5,55 g L-1 i koncentracijama mravlje kiseline većim od 0,4 g L-1 nije zabilježen rast plijesni M. isabellina.

Abstract (english)

Fungus Mortierella isabellina is a potential candidate for lipid production from lignocellulosic hydrolysates. Numerous by-products, which significantly reduce cellulase activity, microbial growth and product accumulation, are formed during lignocellulosic biomass pretreatment. The effect of individual aliphatic acids on growth and lipid accumulation during the submerged cultivation of fungus using glucose as a carbon source was studied. The results showed that the inhibitory effect of formic acid was stronger compared to levulinic acid. Significant drop in both biomass and lipid concentration was observed at levulinic acid concentrations above 1,111 g L-1. It was found that formic acid at lower concentrations promotes biomass growth and lipid biosynthesis. There was no growth of fungus M. isabellina detected at the concentrations of levulinic and formic acid above 5,55 g L-1 and 0,4 g L-1, respectively.

• Keywords: levulinska kiselina; lignocelulozna sirovina; Mortierella isabellina; mravlja kiselina; predobrada
• Keywords (english): formic acid; levulinic acid; lignocellulosic biomass; Mortierella isabellina; pretreatment
• Language: croatian
• URN:NBN: urn:nbn:hr:159:010312
• Study programme: Title: Biotechnology; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2017-09-20 08:36:53