Galvanski članak s katalitičkom elektrodom selektivnom na vodikov peroksid kao platforma za autonomne biosenzore

undergraduate thesis

• Title (english): Galvanic cell with catalytic cathode as a platform for self-powered autonomous biosensors
• Author: Martin Sačer
• Mentor: Damir Iveković (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Chemistry and Biochemistry); (Laboratory for General and Inorganic Chemistry and Electroanalysis); Zagreb
• Defense date and country: 2017-09-19, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Food Technology

Abstract

Enzimski biosenzori danas predstavljaju alternativu već ustaljenim načinima kvalitativne i kvantitavine analize poput titracije, spektrofometrije, kromatografije i dr. Amperometrijski senzori omogućuju visoku selektivnost prema analitu, a njihova primjenjivost mogla bi se još više proširiti izvedbama senzora koji ne zahtjevaju posebno električno napajanje, već se električna struja u njima generira kao posljedica detekcije analita. Polazeći od toga, cilj ovog rada bio je konstruirati galvanski članak s katodom selektivnom na vodikov peroksid, koji bi u kombinaciji s prikladnom oksidazom mogao funkcionirati kao autonoman biosenzor koji u svom radu ne zahtijeva vanjsko električno napajanje. U tu je svrhu napravljena vodljiva tinta modificirana sa željezovim(III) heksacijanoferatom(II) i deponirana na grafitnu elektrodu koja je funkcionirala kao katoda, dok je anoda galvanskog članka načinjena od samoljepljive aluminijske folije. Elektrokemijska svojstva katode i anode istražena su cikličkom voltametrijom te je određen odziv konstruiranog članka pri različitim koncentracijama vodikova peroksida.

Abstract (english)

Enzyme biosensors today stand as the alternative to the more traditional ways of qualitative and quantitative food analysis, such as titration, spectrophotometry, chromatography and others. While using amperometric biosensors, high analyte selectivity is provided, but the applicability of biosensors would be expanded even more, if they could be constructed as autonomous devices that do not require external sources of electricity. One of the possible ways to achieve this goal is to construct biosensors in which the electricity is generated as a part of the analyte detection and determination process. In line with this, the purpose of this work was to construct a galvanic cell with cathode selective to hydrogen peroxide, which could be, in conjunction with appropriate oxidase enzyme, employed as an autonomous, self-powered biosensor. A conducting ink modified with iron(III) hexacyanoferrate(II) was developed and applied in construction of galvanic cell cathode, while the cell anode was fabricated from adhesive aluminum foil. Electrochemical properties of thus fabricated anode and cathode were determined by cyclic voltammetry, and potential response of the constructed galvanic cell towards hydrogen peroxide was evaluated.

• Keywords: amperometrijski biosenzor; berlinsko modrilo; katalitička katoda
• Keywords (english): amperometric biosensors; Prussian blue; catalytic cathode
• Language: croatian
• URN:NBN: urn:nbn:hr:159:744028
• Study programme: Title: Food Technology; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka prehrambene tehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka prehrambene tehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-10-11 10:25:18