Razvoj i validacija novog tipa HPLC detektora za određivanje bioaktivnih sastojaka u hrani

master's thesis

• Title (english): Development and validation of new type OF HPLC detector for determination of bioactive compounds in food
• Author: Vedrana Mandić
• Mentor: Damir Iveković (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Chemistry and Biochemistry); (Laboratory for General and Inorganic Chemistry and Electroanalysis); Zagreb
• Defense date and country: 2017-07-20, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Interdisciplinary Technical Sciences

Abstract

Cilj ovog istraživanja bio je razviti i validirati novi tip HPLC detektora za određivanje bioaktivnih sastojaka u hrani, u ovom slučaju antioksidansa. Kromatografski sustav dizajniran je na način da se ''T-spojnicom'' i posebno izrađenom reakcijskom petljom povezao spektrometrijski (DAD) detektor HPLC sustava s elektrokemijskim biamperometrijski detektorom. Kako bi se utvrdila korelacija signala DAD detektora sa signalom biamperometrijskog detektora, istovremeno su bilježeni odzivi s oba detektora. Princip rada razvijenog elektrokemijskog detektora temeljio se na reakciji polifenola separiranih na HPLC koloni s jako obojenim ABTS (2,2'-azino-bis(3-etilbenzotiazolin-6- sulfonska kiselina)) radikalom te biamperometrijskom mjerenju smanjenja koncentracije ABTS radikala uslijed spomenute reakcije. Odzivni signali oba detektora pokazali su se visoko linearni i korelirani. Primjenjivost razvijenog detektora u analizi realnih uzoraka ispitana je na primjeru određivanja polifenolnih antioksidansa u ekstra djevičanskom maslinovom ulju. Na temelju dobivenih rezultata dane su smjernice za daljnji razvoj biamperometrijskog detektora.

Abstract (english)

The aim of this study was to develop and validate a new type of HPLC detector for determination of bioactive compounds in food, particularly antioxidants. The chromatographic system was designed by connecting the spectrophotometric (DAD) detector of the HPLC system with a biamperometric detector through a ''T-coupler'' and custom designed reaction loop. In order to correlate the signals from DAD and biamperometric detector, data from both detectors were recorded simultaneously. The operating principle of the developed biamperometric detector relied on the reaction between polyphenols separated on the HPLC column and highly-colored ABTS (2,2'-azinobis( 3-etilbenzotiazolin-6-sulfonic acid)) radicals, concentration of which was monitored biamperometrically. Signal responses from both detectors were found to be highly linear and well correlated. The applicability of the developed biamperometric detector in analysis of real samples was tested by the determination of polyphenolic antioxidants in extra virgin olive oil. Based on the results obtained in this work, some guidelines for further development of biamperometric detector were given.

• Keywords: HPLC detektor; antioksidansi; biamperometrijski detektor; ABTS; polifenoli
• Keywords (english): HPLC detector; antioxidant; biamperometric detector; ABTS; polyphenol
• Language: croatian
• URN:NBN: urn:nbn:hr:159:219408
• Study programme: Title: Food Safety Management; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka upravljanja sigurnošću hrane (magistar/magistra inženjer/inženjerka upravljanja sigurnošću hrane)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2017-11-06 13:17:17