Razvoj i validacija HPLC metode za određivanje nitrata u povrću i dječjoj hrani

professional thesis

• Title (english): Development and validation of HPLC method for determination of nitrates in vegetables and baby food
• Author: Danijela Rupić
• Mentor: Ksenija Marković (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; Zagreb
• Defense date and country: 2013-07-24, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Food Technology

Abstract

Cilj ovog rada bio je prikazati razvoj i validaciju HPLC metode za određivanje nitrata u povrću i dječjoj hrani. Nitrati su grupa spojeva koji se mogu naći kao kontaminanti u povrću i dječjoj hrani. Povećanim uzimanjem nitrata/nitrita u organizam dolazi do rizika od razvoja karcinoma zbog nastajanja kancerogenog nitrozamina. Posebno ugrožena skupina jesu dojenčad i mala djeca kod kojih može doći do methemoglobinemije kao posljedice povećanog unosa nitrata u organizam. Methemoglobinemija može završiti fatalno kod male djece i novorođenčadi. Tijekom istraživanja određene su izvedbene značajke validacije i prikazano je sudjelovanje u međulaboratorijskoj usporedbi čime je dokazano da je metoda prikladna za navedenu svrhu i da daje analitički pouzdane rezultate. Analizom uzoraka izuzetih iz trgovačke mreže utvrđeno je da je količina nitrata u povrću i dječjoj hrani u skladu sa važećim Pravilnikom.

Abstract (english)

The purpose of this work was to analyze the development and validation of HPLC method for the determination of nitrates in vegetables and baby food. Nitrates are a group of compounds that can be found as contaminants in vegetables and baby food. Increased intake of nitrates / nitrites in the body leads to the risk of developing cancer due to the formation of carcinogenic nitrosamines. Particularly vulnerable groups are infants and young children in which can lead to methemoglobinemia caused by increased nutrient loads to the body. Methemoglobinemia can be fatal in young children and infants. In this work, the performance characteristics and validation is shown to participate in interlaboratory comparison which indicated that the method is suitable for the intended purpose and that it gives reliable analytical results. Analysis of samples excluded from the trade network showed that the levels of nitrates in vegetables and baby food are in accordance with the Regulation.

• Keywords: nitrati; HPLC; povrće; dječja hrana; izvedbene značajke; validacija; međulaboratorijska usporedba
• Keywords (english): nitrates; HPLC; vegetables; baby food; performance characteristics; validation; interlaboratory comparison
• Language: croatian
• URN:NBN: urn:nbn:hr:159:444817
• Study programme: Title: Postgraduate Specialist Studies in Food Quality and Safety; Study programme type: university; Study level: postgraduate specialist; Academic / professional title: sveučilišni/a specijalist/specijalistica kvalitete i sigurnosti hrane (sveučilišni/a specijalist/specijalistica kvalitete i sigurnosti hrane)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2018-05-17 09:02:05