Preživljavanje bakterije Gluconobacter oxydans u probavnom sustavu miša u prisutnosti patulina i citrinina

undergraduate thesis

• Title (english): Survival of bacterium Gluconobacter oxydans in the digestive system of the mouse in the presence of patulin and citrinin
• Author: Nikolina Kerep
• Mentor: Ksenija Markov (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for General Microbiology and Food Microbiology); Zagreb
• Defense date and country: 2013-09-06, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Bakterija Gluconobacter oxydans je gram negativna bakterija koja zbog svoje izuzetne prilagodbe na ekstremne uvjete rasta, predstavlja idealan organizam u razvoju mikrobnih procesa. Stoga je svrha ovog rada bila izolirati i mikrobiološkim testovima potvrditi bakteriju Gluconobacter oxydans1J i istražiti njezino preživljavanje u probavnom sustavu miša uz prisutnost mikotoksina patulina i citrinina. Neizravnom (posrednom) metodom određivanja broja živih stanica praćeno je preživljavanje bakterije G. oxydans 1J u fecesima miša hranjenih patulinom i citrininom tijekom 14 dana i u homogenatima tankog i debelog crijeva nakon žrtvovanja životinja 8. i 14. dan. Rezultati istraživanja pokazuju da bakterija G. oxydans 1J ima veliku sposobnost preživljavanja u ekstremnim uvijetima, kao što je probavni sustav miša, kao i u prisutnosti patulina i citrinina toksičkih antibiotika širokog spektra.

Abstract (english)

Bacteria Gluconobacter oxydans is a gram negative bacteria which due to its exceptional adaptation to extreme conditions of growth, represents an ideal organism in the development of microbial processes. Because of that, the aim of this study was to isolate and do microbiological tests to confirm the bacterium Gluconobacter oxydans 1J and also explore its survival in the digestive system of mouse in the presence mycotoxin patulin and citrinin. We used indirect method for determination viable cells while we monitored survival of G. oxydans 1J in faeces of mouse fed with patulin and citrinin for 14 days in homogenates of small and large intestine after sacrificing the animals 8th and 14th day. The results show that bacteria G. oxydans 1J has a great ability to survive in extreme conditions, such as the digestive system mouse, as well as in the presence of patulin and citrinin which are toxic broad-spectrum antibiotics.

• Keywords: Gluconobacter oxydans; patulin; citrinin
• Keywords (english): Gluconobacter oxydans; patulin; citrinin
• Language: croatian
• URN:NBN: urn:nbn:hr:159:601772
• Study programme: Title: Biotechnology; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-05-03 12:48:37