Djelovanje 17α-etinilestradiola i dietilstilbestrola na Channel Catfish Ovary (CCO) stanice prilagođene na rast u mediju bez seruma

master's thesis

• Title (english): Effects of 17α-ethynylestradiol and diethylstilbestrol on Channel Catfish Ovary (CCO) cells adapted to growth in serum-free medium
• Author: Marina Srzić
• Mentor: Višnja Gaurina Srček (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Cell Technology, Application and Biotransformations); Zagreb
• Defense date and country: 2012-09-28, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Za ispitivanje učinaka endokrinih modulatora koji su prisutni u okolišu sve više se rabe in vitro testovi toksičnosti. Pri ispitivanju in vitro citotoksičnosti, stanice se najčešće uzgajaju u mediju kojem je dodan serum životinjskog podrijetla koji je bogat faktorima rasta i proteinima, no u novijim istraživanjima provode se testovi u mediju bez seruma. Cilj ovog rada bio je ispitati učinak 17α-etinilestradiola (EE2) i dietilstilbestrola (DES) na CCO stanice koje su prilagođene na rast u mediju bez seruma (CCO-SF). Stanice su tretirane EE2 i DESom u koncentracijama od 0,1 do 10 µg/mL u UC mediju bez seruma. Tijekom 72 sata od tretmana, vijabilnost CCO-SF stanica određena je WST-1 metodom. Na temelju dobivenih rezultata i usporedbe EC50vrijednosti s vrijednostima za CCO stanice uzgojenim u mediju sa serumom pokazano je da je citotoksični učinak EE2 i DES-aizraženiji u mediju sa serumom.

Abstract (english)

The in vitro tests are frequently used to determine the impact of endocrine modulators present in environment. During the in vitro testing of citotoxicity, the cells are grown in the media with added serum of animal origin, which contains growth factors and proteins, but recent research are conducted in serum-free conditions. The aim of this study was to investigate the effect of 17α-ethynylestradiol (EE2) and diethylstilbestrol (DES) on CCO cells cultivated in comercial serum-free medium. Cells were treated with EE2 and DES and EE2 in concentration between 0,1 and 10 µg/ml in UC medium without serum. During 72 hours of treatment, the viability of CCO cells has been determined by WST-1 method. The obtained results and comparison with those gained with CCO cells grown in compleated medium with serum showed that cytotoxic effects of EE2 and DES is more pronounced in medium with serum added.

• Keywords: CCO stanice; dietilstilbestrol; 17α-etinilestradiola; medij bez seruma
• Keywords (english): CCO cells; diethylstilbestrol; 17α-ethynylestradiol; serum- free medium
• Language: croatian
• URN:NBN: urn:nbn:hr:159:104089
• Study programme: Title: Molecular Biotechnology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-05-09 11:02:03