Amperometrijski biosenzor za glukozu s glukoza oksidazom i peroksidazom imobiliziranom na površinu titanatnih nanocjevčica

master's thesis

• Title (english): Amperometric glucose biosensor with glucose oxidase and peroxidase immobilized on the surface of titanate nanotubes
• Author: Dijana Begić
• Mentor: Damir Iveković (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Chemistry and Biochemistry); (Laboratory for General and Inorganic Chemistry and Electroanalysis); Zagreb
• Defense date and country: 2012-12-21, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Biosenzor je vrsta kemijskog senzora, koji se temelji na direktnoj vezi imobilizirane biološki aktivne tvari s prikladnim pretvornikom signala. Cilj ovog rada bio je izraditi i ispitati amperometrijski biosenzor na glukozu temeljen na glukoza oksidazi i amperometrijskom pretvorniku s peroksidazom imobiliziranom na titanatne nanocjevčice. Istraženi su optimalni uvjeti rada biosenzora i utvrđene njegove analitičke karakteristike. Razvijeni biosenzor pokazao se primjenjivim u analizi glukoze u realnim uzorcima voćnih sokova i izotoničnih napitaka.

Abstract (english)

Biosensor is a type of chemical sensor which is based on the direct connection between the immobilized biologically active receptor and the suitable transducer. The aim of this study was to develop and test the novel type of amperometric glucose biosensor based on glucose oxidase and an amperometric transducer with peroxidase immobilized on the surface of titanate nanotubes. Optimal values of working parameters and analytical performances of developed biosensor were determined. It was found that the biosensor is applicable for glucose determination in fruit juices and isotonic beverages.

• Keywords: glukoza oksidaza; amperometrijski biosenzor; titanatne nanocjevčice; peroksidaza; vodikov peroksid
• Keywords (english): glucose oxidase; amperometric biosensor; titanatne nanotubes; peroxidase; hydrogen peroxide.
• Language: croatian
• URN:NBN: urn:nbn:hr:159:735786
• Study programme: Title: Biotechnology; specializations in: Biochemical Engineering; Course: Biochemical Engineering; Study programme type: university; Study level: graduate; Academic / professional title: diplomirani inženjer biotehnologije; smjer biokemijsko inženjerstvo (diplomirani inženjer biotehnologije; smjer biokemijsko inženjerstvo)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-05-17 11:17:44