Učinak 17α-etinilestradiola na CCO i CHO-K1 stanice te primarnu kulturu ovarija štakora

master's thesis

• Title (english): Effect of 17α-ethynylestradiol on CCO and CHO-K1 cells and primary cultures of rat ovarian cells
• Author: Mirna Mihajlović
• Mentor: Višnja Gaurina Srček (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Cell Technology, Application and Biotransformations); Zagreb
• Defense date and country: 2010-03-05, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Kulture životinjskih stanica važne su u toksikološkim istraživanjima gdje se kao in vitro modeli koriste pri ispitivanjima učinaka različitih ksenobiotika. U području ekotoksikologije intenzivno se provode istraživanja utjecaja endokrinih modulatora, kemijskih spojeva koji oponašaju prirodne hormone i ometaju njihovo djelovanje u organizmu. Utvrđeno je da vrlo niske koncentracije kako prirodnih, tako i sintetskih estrogena prisutnih u okolišu ometaju rad endokrinog sustava te uzrokuju značajne promjene u reproduktivnom sustavu životinja i ljudi. 17α-etinilestradiol (EE2) je sintetski derivat prirodnog hormona estradiola i koristi se kao glavna komponenta oralnih kontraceptiva. Cilj ovog rada bio je odrediti citotoksični učinak EE2 na rast i morfološke promjene stanične linije CCO (Channel Catfish Ovary) i CHO-K1 (Chinese Hamster Kidney) te primarne kulture ovarija štakora. Usporedbom učinka EE2 na dvije stanične linije možemo zaključiti da je stanična linija CCO osjetljivija na djelovanje EE2 nego stanična linija CHO-K1. U obje stanične linije zapažen je pozitivan učinak na rast stanica pri niskim koncentracijama EE2 (0,01-0,1 μg/mL), što je u literaturi opisano kao pojava tzv. kemijske hormeze, dok su više koncentracije (5-50 μg/mL) imale citotoksičan učinak. Kod primarne kulture stanica ovarija štakora sve ispitane koncentracije EE2 djeluju inhibitorno na rast stanica pri čemu više koncentracije (1-20 μg/mL) pokazuju slabiji citotoksični učinak nego kod CCO i CHO-K1 stanica. U svim ispitanim kulturama vidljive su morfološke promjene u izgledu stanica izloženih djelovanju EE2.

Abstract (english)

Animal cell cultures are important element in toxicological research where they are used as in vitro model for testing of different xenobiotics. Over recent years there has been intensive research of the influence of endocrine modulators, chemical substances that imitate natural hormones and influence their effects in organism causing pathological changes both humans and animals. It has been determined that very low concentration as well as natural and synthetic estrogen present in the environment disturb normal function of endocrine system and cause significant changes in reproductive system both humans and animals. 17α-ethynylestradiol (EE2) is synthetic derivate of natural hormone estradiol which is used as main component of oral contraceptives. The objective of this study was to determine cytotoxic effects of EE2 on the growth and morphological changes of cell lines CCO (Channel Catfish Ovary) and CHO-K1 (Chinese Hamster Kidney) and primary culture of rat's ovaries. In comparison of effect of EE2 on two cell lines we can conclude that CCO cell line is more sensitive CHO-K1 cell line. In both cell lines it was noticed stimulatory effect of low concentrations of EE2 which is described as chemical hormesis, while higher concentrations of EE2 had cytotoxic effect. In primary cultures of rat ovarian cells lower concentrations of EE2 inhibited cell growth while higher concentrations showed weaker cytotoxic effect then in CCO and CHO-K1 cells. All cultures had morphological changes in appearance of EE2 treated cells.

• Keywords: CCO stanice; CHO-K1 stanice; citotoksičnost; 17α-etinilestradiol; primarna kultura
• Keywords (english): CCO cells; CHO-K1 cells; cytotoxicity; 17α-ethynylestradiol; primary culture
• Language: croatian
• URN:NBN: urn:nbn:hr:159:778185
• Study programme: Title: Biotechnology; specializations in: Biochemical Engineering; Course: Biochemical Engineering; Study programme type: university; Study level: graduate; Academic / professional title: diplomirani inženjer biotehnologije; smjer biokemijsko inženjerstvo (diplomirani inženjer biotehnologije; smjer biokemijsko inženjerstvo)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-08-28 09:32:40