Citotoksički učinak PCB-77 na profileraciju CHO(Chinese Hamster Ovary)-K1 stanica

master's thesis

• Title (english): Cytotoxic effect of PCB-77 on proliferation of CHO(Chinese Hamster Ovary)-K1 cells
• Author: Jelena Muštra
• Mentor: Jasna Kniewald (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Chemistry and Biochemistry); (Laboratory for Toxicology); Zagreb
• Defense date and country: 2008-12-12, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Food Technology

Abstract

Korištenje polikloriranih bifenila u otvorenim sustavima zabranjeno je još 70-ih godina prošlog stoljeća, jer su dokazana mnoga negativna djelovanja na živi svijet. Unatoč tome i danas ih je moguće pronaći u okolišu zbog izrazite perzistentnosti. Toksički učinci raznih ksenobiotika određuju se provođenjem klasičnih (in vivo) i alternativnih (in vitro) testova. U ovom radu kao test-sustav za praćenje citotoksičkog učinka PCB-77 primijenjena je stanična linija ovarija kineskog hrčka (CHO-K1) u monosloju, uzgojena u Dulbecco MEM mediju uz dodatak 5% FBS (Foetal Bovine Serum). Citotoksički učinak ispitan je pri koncentracijama 5 - 100 μM PCB-77 korištenjem Trypan Blue metode tijekom 24, 48 i 72 sata. Nakon 72 sata inkubacije inhibicija proliferacije CHO-K1 stanica iznosila je 17,5 – 84,3 % pri 5 - 50 μM koncentracijama PCB-77, te je uočena linearnost inhibicije s povećanjem doze PCB-77. IC vrijednosti su izračunate iz jednadžbi interpoliranih eksponencijalnih krivulja s grafičkog prikaza ovisnosti % inhibicije proliferacije stanica pri korištenim koncentracijama PCB-77. IC50 vrijednost za PCB-77 iznosi 15,11 μM nakon 72 sata. Primijenjena metoda (in vitro eksperimentalni uvjeti) pokazala se jednostavnom i brzom za izvođenje te se može koristiti u određivanju citotoksičkih učinaka i drugih ksenobiotika.

Abstract (english)

The use of polychlorinated biphenyls (PCBs) was restricted in the early 1970s and ultimately ended in 1977 because of their adverse effects on human health. However, it is still possible to find them in the environment because of their high persistance. Toxic effects of xenobiotics can be determined by application of classical (in vivo) and alternative (in vitro) toxicity tests. In this work the hamster ovary (CHO-K1) cell line in monolayer cultivated in Dulbecoo's MEM medium supplemented with 5% FBS (Foetal Bovine Serum) was applied as the test system for cytotoxicity determination of PCB-77. The cytotoxic effect of 5 – 100 μM PCB-77 was followed by Trypan Blue method during 24, 48 and 72 hours. After 72 hours the inhibition of CHO-K1 cells proliferation at 5 – 50 μM PCB-77 was 17,5 – 84,3 %. The inhibition of proliferation was proportional to the increase of the concentration of PCB-77 and the incubation time. IC values were derived from to the equations of related exponential trend lines interpolated in the graphically presented correlation between % of cell growth inhibition and applied PCB-77 concentrations. IC50 for PCB-77 after 72 hours was 15,11 μM. The used method (in vitro experimental conditions) was proved as simple and fast, and can be applied in the cytotoxicity studies of different xenobiotics.

• Keywords: CHO (Chinese Hamster Ovary)-K1 stanična linija; PCB-77; citotoksičnost; alternativni in vitro test
• Keywords (english): CHO (Chinese Hamster Ovary)-K1 cell line; PCB-77; cytotoxicity; alternative in vitro test
• Language: croatian
• URN:NBN: urn:nbn:hr:159:358755
• Study programme: Title: Food Technology; specializations in: Nutrition; Course: Nutrition; Study programme type: university; Study level: graduate; Academic / professional title: diplomirani inženjer prehrambene tehnologije; smjer nutricionizam (diplomirani inženjer prehrambene tehnologije; smjer nutricionizam)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-08-31 09:55:00