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PDF 1.68 MB
master's thesis
10
4
Biochemical and biophysics studies of cell wall of industrial yeasts
Danijela Zeljko (2016)
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Chemistry and Biochemistry
Laboratory for Biochemistry
Cite this item:
https://urn.nsk.hr/urn:nbn:hr:159:788910
Metadata
Title
Biokemijska i fizikalna analiza stanične stijenke kvasca
Author
Danijela Zeljko
Mentor(s)
Vladimir Mrša
(thesis advisor)
Abstract
Cilj ovog rada je bio odrediti udio komponenti stanične stijenke kvasca Saccharomyces cerevisiae: manoproteina, β-1,3-glukana, β-1,6 glukana i hitina, koristeći novo razvijenu metodu koja se temelji na enzimskim i kemijskim metodama. Ova metoda je testirana na različitim sojevima kvasca i došlo se do zaključka da uvjeti rasta imaju utjecaj na udio staničnih komponenti. Metoda je korištena za procjenu sastava stanične stijenke industrijskih sojeva (autolizirani i osušeni sojevi) koji su bili pod utjecajem različitih fermentacijskih i drugih procesa. Korišteni su i fenotipski testovi da se uoči i potvrdi da rast različitih sojeva ovisi o sastavu hranjive podloge. U drugom dijelu rada je promatran utjecaj etanol stresa na rast te na udio komponenti stanične stijenke različitih laboratorijskih sojeva kvasca.
Keywords
chitin
ethanol
Saccharomyces cerevisae
cell wall
β-glucan
Parallel title (English)
Biochemical and biophysics studies of cell wall of industrial yeasts
Granter
University of Zagreb
Faculty of Food Technology and Biotechnology
Lower level organizational units
Department of Chemistry and Biochemistry
Laboratory for Biochemistry
Place
Zagreb
State
Croatia
Scientific field, discipline, subdiscipline
BIOTECHNICAL SCIENCES
Biotechnology
Study programme type
university
Study level
graduate
Study programme
Molecular Biotechnology
Academic title abbreviation
mag. ing. biotechn.
Genre
master's thesis
Language
English
Defense date
2016-10-11
Parallel abstract (English)
The aim of this study was to quantify the amount of specific components in the cell wall of yeast Saccharomyces cerevisiae, namely mannoproteins, β-1,3-glucans and β-1,6-glucans and chitin using newly developed enzymatic and chemical method. These new methods were validated on various cell wall mutants and it was observed that growth conditions have strong effects on the amount of cell wall components. This method was used to evaluate cell wall composition of industrial yeasts (autolysed and dried yeast) that were subjected to different fermentation and treatment processes. The phenotypic assay was used to confirm and to notice how the growth of various strains depends on composition of medium. The second part of the project was to examine the effect of ethanol stress on cell wall composition and to analyze cell wall composition in industrial yeast cells, to determine whether culture/process conditions have an impact on cell wall composition.
Parallel keywords (Croatian)
etanol
hitin
Saccharomyces cerevisae
stanična stijenka
β-glukan
Resource type
text
Access condition
Open access
Terms of use
URN:NBN
https://urn.nsk.hr/urn:nbn:hr:159:788910
Committer
Jelena Viličić