Biochemical and biophysics studies of cell wall of industrial yeasts

master's thesis

• Title: Biokemijska i fizikalna analiza stanične stijenke kvasca
• Author: Danijela Zeljko
• Mentor: Vladimir Mrša (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Chemistry and Biochemistry); (Laboratory for Biochemistry); Zagreb
• Defense date and country: 2016-10-11, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Cilj ovog rada je bio odrediti udio komponenti stanične stijenke kvasca Saccharomyces cerevisiae: manoproteina, β-1,3-glukana, β-1,6 glukana i hitina, koristeći novo razvijenu metodu koja se temelji na enzimskim i kemijskim metodama. Ova metoda je testirana na različitim sojevima kvasca i došlo se do zaključka da uvjeti rasta imaju utjecaj na udio staničnih komponenti. Metoda je korištena za procjenu sastava stanične stijenke industrijskih sojeva (autolizirani i osušeni sojevi) koji su bili pod utjecajem različitih fermentacijskih i drugih procesa. Korišteni su i fenotipski testovi da se uoči i potvrdi da rast različitih sojeva ovisi o sastavu hranjive podloge. U drugom dijelu rada je promatran utjecaj etanol stresa na rast te na udio komponenti stanične stijenke različitih laboratorijskih sojeva kvasca.

Abstract (english)

The aim of this study was to quantify the amount of specific components in the cell wall of yeast Saccharomyces cerevisiae, namely mannoproteins, β-1,3-glucans and β-1,6-glucans and chitin using newly developed enzymatic and chemical method. These new methods were validated on various cell wall mutants and it was observed that growth conditions have strong effects on the amount of cell wall components. This method was used to evaluate cell wall composition of industrial yeasts (autolysed and dried yeast) that were subjected to different fermentation and treatment processes. The phenotypic assay was used to confirm and to notice how the growth of various strains depends on composition of medium. The second part of the project was to examine the effect of ethanol stress on cell wall composition and to analyze cell wall composition in industrial yeast cells, to determine whether culture/process conditions have an impact on cell wall composition.

• Keywords: etanol; hitin; Saccharomyces cerevisae; stanična stijenka; β-glukan
• Keywords (english): chitin; ethanol; Saccharomyces cerevisae; cell wall; β-glucan
• Language: english
• URN:NBN: urn:nbn:hr:159:788910
• Study programme: Title: Molekularna biotehnologija; Study programme type: sveučilišni; Study level: diplomski; Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2017-04-25 11:12:01