Biokemijska i bioloŠka karakterizacija Granzima K i njegova biološka uloga u sistemskoj upali / sepsi

doctoral thesis

• Title (english): BIOCHEMICAL AND BIOLOGICAL CHARACTERIZATION OF GRANZYME K AND ITS BIOLOGICAL ROLE IN SYSTEMIC INFLAMMATION / SEPSIS
• Author: Marijana Ručević; MBZ: 241373
• Mentor: Vladimir Mrša (mentor); MBZ: 39525
• Committee member: Đuro Josić (predsjednik povjerenstva); MBZ: 307736
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; Zagreb
• Defense date and country: 2007-09-24, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology
• Universal decimal classification (UDC): 60 - Biotechnology. Genetic engineering. Products of cloning

Abstract

U ovom je radu razvijen kompetitivni Granzim K (GrK) specifični imunotest s ciljem da se istraži uloga te proteaze u sistemskoj upali/sepsi. Utvrđeno je značajno povećanje koncentracije GrK u plazmi pacijenata hitnog odjela s pretpostavljenom dijagnozom sepse, dok je u plazmi pacijenata uključenih u klinički pokus s dijagnosticiranom sepsom utvrđeno značajno smanjenje koncentracije GrK, u usporedbi s kontrolnom skupinom. Slobodni GrK s molekulskom težinom od ~26 kDa uočen je samo u plazmi pacijenata sa sepsom. Rezultati upućuju da bi GrK mogao biti dio patofiziologije sepse te na potencijalnu mogućnost njegova korištenja kao dijagnostičkog biomarkera za stupnjevanje sepse. U radu je započeta i karakterizacija GrK kako bi se razjasnila biološke uloga te moćne proteaze u sistemskoj upali/sepsi.

Abstract (english)

The aim of this study was to investigate the potential role of Granzyme K (GrK) in systemic inflammation/sepsis and therefore a novel GrK-specific immunoassay was developed. The plasma levels of GrK were found increased in patients admitted to the emergency department with a putative diagnosis of sepsis while decreased in patients with severe sepsis enrolled in a clinical trial, compared to healthy subjects. The free GrK as a 26 kDa protein appeared only in patients with sepsis. The results indicate that plasma GrK levels could serve as a useful diagnostic marker to stage sepsis and may play a functional role in the development of sepsis. Characterization of human GrK was also initiated in order to elucidate biological function(s) of this potent serine protease in inflammatory responses/sepsis.

• Keywords: Biomarker; Inter-alfa inhibitor proteini; Granzimi; Granzim K; Sepsa; Serinske proteaze
• Keywords (english): Biomarkers; Inter-alpha inhibitor proteins; Granzymes; Granzyme K; Sepsis; Serine Protease
• Language: croatian
• URN:NBN: urn:nbn:hr:159:492961
• Promotion: 2008-09-28
• Study programme: Title: Postgraduate PhD Study in Biotechnology and Bioprocess Engineering; Study programme type: university; Study level: postgraduate; Academic / professional title: doktor/doktorica znanosti (doktor/doktorica znanosti)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2018-05-11 12:53:06