Određivanje citotoksičnog i antioksidacijskog učinka ekstrakata listova bobičastog voća

undergraduate thesis

• Title (english): Determination of the cytotoxic and antioxidant effect of berry leaf extracts
• Author: Marija Čaljkušić
• Mentor: Ana Huđek Turković (mentor)
• Mentor: Ksenija Durgo (pomoć pri izradi, neposredni voditelj)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Biology and Microbial Genetics); Zagreb
• Defense date and country: 2024-09-16, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Bobičasto voće spada među najbolje prehrambene izvore biološki aktivnih spojeva s pozitiv nim učinkom na zdravlje. Njihova industrijska prerada rezultira značajnom količinom otpada u obliku lišća koje obiluje polifenolima s dokazanim antioksidacijskim i protuupalnim djelovanjem. Cilj ovog rada bio je ispitati biološki učinak ekstrakata listova aronije, borovnice i crnog ribizla na in vitro staničnim linijama skvamoznog epitela karcinoma jezika CAL 27 i hepatocelularnog karcinoma jetre HepG2. Ispi tivanje utjecaja na stanično preživljenje i klonogeni rast provedeno je MTT i klonogenim testom. Metoda DCFH-DA korištena je u svrhu ispitivanja unutarstanične indukcije slobodnih radikala. Najveći biološki učinak pokazao je ekstrakt lista crnog ribizla koji u cijelom rasponu koncentracija smanjuje preživljenje obje ispitivane stanične linije na ~ 80% pri čemu pokazuje i jak dozno-ovisni inihibitorni učinak na njihov klonogeni rast. S druge strane, ekstrakt lista aronije pokazuje proliferativno i prooksidativno djelovanje na HepG2 staničnu liniju. Borovnica nije pokazala značajnije biološke učinke na ispitivane test sustave.

Abstract (english)

Berries are one of the best food sources for biologically active compounds that have a posi tive effect on health. Their industrial processing results in a significant amount of waste in the form of leaves that are rich in polyphenols with proven antioxidant and anti-inflammatory effects. The aim of this work was to examine the biological effect of chokeberry, blueberry, and blackcurrant leaf extracts on the in vitro squamous epithelial cell lines of tongue carcinoma CAL 27 and hepatocellular carcinoma of the liver HepG2. Testing of the influence on cell survival and clonogenic growth was performed by MTT and clonogenic assay. The DCFH-DA method was used to examine the intracellular induction of free radicals. The greatest biological effect was shown by blackcurrant leaf extract, which in the entire range of concentrations reduces the survival of both tested cell lines to ~ 80% while also showing a strong dose-dependent inhibitory effect on their clonogenic growth. On the other hand, chokeberry leaf extract shows a proliferative and slightly prooxidative effect on the HepG2 cell line. Blueberry did not show significant biological effects on the examined test systems.

• Keywords: ekstrakt listova bobičastog voća; citotoksičnost; antioksidacijski potencijal; klonogeni rast; in vitro biološki test sustavi
• Keywords (english): berry leaf extract; cytotoxicity; antioxidant potential; clonogenic growth; in vitro biological test systems
• Language: croatian
• URN:NBN: urn:nbn:hr:159:398202
• Study programme: Title: Biotechnology; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2024-09-26 07:53:09