Utjecaj UVC zračenja na uzorke biološkog podrijetla

master's thesis

• Title (english): Effect of UVC radiation on biological samples
• Author: Dario Zrnec
• Mentor: Višnja Bačun-Družina (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Biology and Microbial Genetics); Zagreb
• Defense date and country: 2012-07-09, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Forenzična genetika bavi se utvrđivanjem DNA profila dobivenih DNA analizom tragova biološkog podrijetla. DNA profili identificirani umnažanjem 15 kratkih uzastopno ponavljajućih polimorfnih lokusa (engl. Short Tandem Repeat, STR) i spolnog biljega amelogenina imaju visoku snagu diskriminacije. U forenzičnim laboratorijima, koriste se komercijalna sredstva za čišćenje i UVC lampe za sprječavanje kontaminacije radnih površina prilikom analize uzoraka biološkog podrijetla. UVC zračenje uzrokuje nastanak ciklobutanskih pirimidinskih dimera (CPD), pirimidin 6-4 pirimidon fotoprodukata (6-4 PP), jednolančanih te dvolančanih lomova DNA. Cilj ovog rada bio je ispitati utjecaj UVC zračenja na uzorke i kontrole izolirane DNA te razrijeđene i nerazrijeđene uzorke i kontrole krvi, sline i sperme, kao i odrediti dozu UVC zračenja pri kojoj nije moguće identificirati DNA profil ispitanika, uslijed degradacije DNA.

Abstract (english)

Forensic genetics deals with determining DNA profiles obtained by DNA analysis of traces of biological origin. DNA profiles identified by amplifying 15 Short Tandem Repeats (STR) loci and a sex marker amelogenin have high discrimination power. In forensic laboratories, commercial cleaning agents and UVC lamps are used for the prevention of contamination of work surfaces during the analysis of samples of biological origin. UVC radiation causes formation of cyclobutane pyrimidine dimers (CPD), pyrimidine 6-4 pirimidone photoproducts (6-4 PP) and single and double stranded DNA brakes. The goal of this research is to examine the effects of UVC radiation on isolated DNA samples and controls, disolved as well as non-dissolved samples and control blood, saliva and sperm samples and to determine the dose of UVC radiation at which identification of the DNA profile of the donor is not possible due to DNA degradation.

• Keywords: uzorci biološkog podrijetla; DNA profil; kontaminacija; UVC zračenje; STR lokus
• Keywords (english): biological samples; DNA profile; contamination; UVC radiation; STR loci
• Language: croatian
• URN:NBN: urn:nbn:hr:159:171481
• Study programme: Title: Molecular Biotechnology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-05-08 12:04:10