Primjena prirodnih eutektičih otapala u ekstrakciji antocijana

undergraduate thesis

• Title (english): Use of natural deep eutectic solvents in extraction of anthocyanins
• Author: Ivana Čulo
• Mentor: Ivana Radojčić Redovniković (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Cell Technology, Application and Biotransformations); Zagreb
• Defense date and country: 2017-09-08, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Food Technology

Abstract

S ciljem zaštite okoliša, povećan je broj istraživanja u kojima se razvijaju zeleni procesi. Takvi procesi, između ostalog, podrazumijevaju korištenje otapala koja nisu štetna za okoliš i ljude koji njima rukuju i zamjena su za hlapljiva organska otapala sa tehnološkim svojstvima istima organskim otapalima. Jedana od alternativnih otapala su prirodna eutektička otapala, smjese 2 ili 3 tvari povezane vodikovim vezama. U ovom radu korišteno je 6 različitih prirodnih eutektičkih otapala za ekstrakciju antocijana iz liofilizirane komine grožđa sorte Plavac mali, a pripremljeni ekstrakti su analizirani tekućinskom kromatografijom visoke djelotvornosti (HPLC). S masenim udjelom po gramu komine većim od zakiseljenog etanola kao konvencionalnim otapalom za ekstrakciju antocijana, najselektivnije otapalo za ekstrakciju antocijana iz komine grožđa bilo je kolin-klorid:limunska kiselina (2550 ± 101,5 μg g-1 s.tv).

Abstract (english)

With the aim of protecting the environment, there is a growing number of researches in which green processes are being developed. Such processes, among other things, imply the use of non-polluting solvents that are replace for volatile organic solvents, which are toxic for environment and people who handle them. An example of alternative solvents are natural deep eutectic solvents, mixtures of 2 or 3 substances bonded with hydrogen bonds. In this experiment, 6 different natural eutectic solvents were used for the extraction of anthocyanins from the lyophilized grape pomace of Plavac mali, and the prepared extracts were analyzed using the high performance liquid chromatography (HPLC). With the weight ratio per gram of pomace higher than the acidified ethanol as a conventional solvent for the extraction of anthocyanins, the most selective solvent in extracting anthocyanins from the grape pomace was choline chloride:citric acid (2550 ± 101.5 μg g-1 s.tv).

• Keywords: antocijani; ekstrakcija; HPLC kromatografija; prirodna eutektička otapala
• Keywords (english): anthocyanins; extraction; HPLC cromatography; natural deep eutectic solvents
• Language: croatian
• URN:NBN: urn:nbn:hr:159:715007
• Study programme: Title: Food Technology; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka prehrambene tehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka prehrambene tehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2017-09-07 16:51:28