Učinci proteinskih hidrolizata iz pogače konoplje na proliferaciju tumorske stanične linije HeLa

undergraduate thesis

• Title (english): The effect of hemp seed protein hydrolysates on the tumour cell line HeLa proliferation
• Author: Lana Mišić
• Mentor: Višnja Gaurina Srček (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Cell Technology, Application and Biotransformations); Zagreb
• Defense date and country: 2018, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Bioaktivni peptidi su prirodne kemijske tvari koje se nalaze u biljkama i životinjama te pozitivno djeluju na ljudsko zdravlje. Zbog visoke specifičnosti i afiniteta za tkivo te pozitivnog učinka na zdravlje, postaju sve zanimljiviji izbor za znanstvena istraživanja, a oslobađaju se tijekom enzimske proteolize proteina (gastrointestinalna digestija ili in vitro hidroliza proteolitičkim enzimima). Hidrolizat proteina iz pogače konoplje pokazao je, u brojnim znanstvenim istraživanjima, da posjeduje multifunkcionalne bioaktivne peptide, primjerice WVYY i PSLPA koji pokazuju antioksidacijski i antihipertenzivni učinak. Cilj ovog rada bio je ispitati učinak hidrolizata proteina iz pogače konoplje, dobivenog djelovanjem s enzimom alkalazom, na proliferaciju HeLa stanica (stanice raka vrata maternice) MTS metodom. Hidrolizom enzimom alkalazom dobiven je ishodni hidrolizat koji sadrži peptide različitih veličina te je ultrafiltracijom odvojena frakcija koja je sadržavala peptide manje od 3 kDa. Ishodni hidrolizat (s peptidima različitih veličina) pri koncentraciji proteina od 5 mg/mL, pokazao je snažno inhibitorno djelovanje na HeLa stanice uz preživljenje od 29,4 %. Hidrolizat s koncentracijom od 1 mg/mL pokazao je blago inhibitorno djelovanje, uz preživljenje stanica od 86,03%. Istovremeno, frakcija hidrolizata s peptidima manjim od 3 kDa, pri svim ispitanim koncentracijama, nije pokazala inhibicijsko djelovanje. Za širu sliku djelovanja proteinskih hidrolizata i njihovih frakcija potrebno je napraviti dodatna istraživanja, kako na HeLa stanicama, tako i na drugim tumorskim stanicama.

Abstract (english)

Bioactive peptides are natural chemical substances found in plants and animals which have positive impact on human health. Because of its high specificity and affinity for tissue and positive impact on health, they become more interesting choice for scientific research and they are released during enzymatic proteolysis of proteins (gastrointestinal digestion or in vitro hydrolysis with proteolytic enzymes). Hemp seed protein hydrolysates has shown, in many scientific researches, that owns multifunctional bioactive peptides, for example WVYY and PSLPA which have shown antioxidant and antihypertensive effect. The aim of this work was to assay the effect of hemp seed protein hydrolysate, obtained by proteolitic enzyme alcalase,on proliferation of HeLa cells (cervical cancer cells) using MTS method. By alcalase proteolysis we got initial hydrolysate which contained peptides various sizes and by ultrafiltration was seperated fraction that contained only peptides smaller then 3 kDa. Initial protein hydrolysate with protein concentration of 5 mg/mL has shown strong inhibitory effect on HeLa cells with survival of 29,4%. Weaker inhibition showed initial hydrolysate with protein concentration of 1 mg/mL with cell survival of 86,03%. At the same time, hydrolysate fraction with peptides smaller than 3 kDa, in all concentrations, did not show inhibitory effect. For a wider picture of protein hydrolysate and its fractions effect, it is necessary to do many more in vitro researches, with HeLa and other cancer cells.

• Keywords: bioaktivni peptidi; HeLa stanice; pogača konoplje; proteinski hidrolizat
• Keywords (english): bioactive peptides; HeLa cells; hemp seed; protein hydrolysate
• Language: croatian
• URN:NBN: urn:nbn:hr:159:335298
• Study programme: Title: Biotechnology; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2018-09-24 11:24:09