Extraction of Phycobiliproteins from Cyanobacteria and Red Algae

diplomski rad

• Autor: Tihana Merčep
• Mentor: Nataša Bauer (mentor)
• Ustanova koja je dodijelila akademski / stručni stupanj: Sveučilište u Zagrebu; Prehrambeno-biotehnološki fakultet; Zagreb
• Datum i država obrane: 2010-07-02, Hrvatska
• Znanstveno / umjetničko područje, polje i grana: BIOTEHNIČKE ZNANOSTI; Biotehnologija

Sažetak

The phycobiliproteins are intensely fluorescent proteins from cyanobacteria and red algae. Because of their optical properties, they are ideal for use as fluorescent labels in medical diagnostics and immunological assays. In this work, 18 species of algae are tested for the presence of phycobiliproteins in order to find the culture that would produce the most of the certain pigment. C-phycocyanin and allophycocyanin are extracted from cyanobacteria. R-phycocyanin, B-phycoerythrin, and b-phycoerythrin are extracted from red algae. High purity of pigments is obtained. It is shown that from tested algae, GS023 would be the most productive for obtaining C-phycocyanin and GS028 would be the most productive for obtaining B-phycoerythrin.

• Ključne riječi: cyanobacteria; red algae; phycocyanin; allophycocyanin; phycoerythrin
• Jezik: engleski
• URN:NBN: urn:nbn:hr:159:788036
• Studijski program: Naziv: Molekularna biotehnologija; Vrsta studija: sveučilišni; Stupanj studija: diplomski; Akademski / stručni naziv: magistar/magistra inženjer/inženjerka molekularne biotehnologije (mag. ing. biotechn.)
• Vrsta resursa: Tekst
• Način izrade datoteke: Izvorno digitalna
• Prava pristupa: Pristup korisnicima matične ustanove
• Datum i vrijeme pohrane: 2017-06-02 12:58:24