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undergraduate thesis
Restrikcijska analiza ekspresijskog plazmida za enzim I-SceI
2010. urn:nbn:hr:159:568058
Gardlo, Nikolina
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biology and Microbial Genetics

Cite this document

Gardlo, N. (2010). Restrikcijska analiza ekspresijskog plazmida za enzim I-SceI (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:568058

Gardlo, Nikolina. "Restrikcijska analiza ekspresijskog plazmida za enzim I-SceI." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2010. https://urn.nsk.hr/urn:nbn:hr:159:568058

Gardlo, Nikolina. "Restrikcijska analiza ekspresijskog plazmida za enzim I-SceI." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2010. https://urn.nsk.hr/urn:nbn:hr:159:568058

Gardlo, N. (2010). 'Restrikcijska analiza ekspresijskog plazmida za enzim I-SceI', Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 07 May 2024, https://urn.nsk.hr/urn:nbn:hr:159:568058

Gardlo N. Restrikcijska analiza ekspresijskog plazmida za enzim I-SceI [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2010 [cited 2024 May 07] Available at: https://urn.nsk.hr/urn:nbn:hr:159:568058

N. Gardlo, "Restrikcijska analiza ekspresijskog plazmida za enzim I-SceI", Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2010. Available at: https://urn.nsk.hr/urn:nbn:hr:159:568058

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Metadata
TitleRestrikcijska analiza ekspresijskog plazmida za enzim I-SceI
Title (english)The restriction analysisof the plasmid encoding I-SceI enzyme
AuthorNikolina Gardlo
MentorIvan-Krešimir Svetec (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Biology and Microbial Genetics)
Zagreb
Defense date and country2010-07-15, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Endonukleaza I-SceI pripada skupini „homing“ endonukleaza, prepoznaje i cijepa asimetričnu sekvenciju DNA duljine od 18 pb. Ovaj enzim je visoko specifičan, zbog čega se često koristi u genetičkom inženjerstvu i genskoj terapiji. U ovom radu plazmidna DNA izolirana je iz soja kvasca Saccharomyces cerevisiae koji je prethodno transformiran plazmidom koji kodira za enzim I-SceI. Rezultati restrikcijske analize potvrdili su prisutnost plazmida pYES-SCE.
Abstract (english)
Endonuclease I-SceI belongs to the group of „homing“ endonucleases, that recognize and cleave asymmetric 18 pb sequence. As this enzyme has a high specificity, it has been used in genetic engineering and gene therapy. In this study plasmid DNA was isolated from the strain of yeast Saccharomyces cerevisiae, previously transformed by plasmid encoding I-SceI enzyme. The restriction analysis of the plasmid confirmed the presence of pYES-SCE plasmid.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:568058
Study programmeTitle: Biotechnology
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2017-06-02 10:51:28