Analiza pentanukleotidnih STR lokusa – hrvatska populacijska studija

master's thesis

• Title (english): Analysis of pentanucleotide STR loci – Croatian population study
• Author: Ivana Heckel
• Mentor: Vladimir Mrša (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Chemistry and Biochemistry); (Laboratory for Biochemistry); Zagreb
• Defense date and country: 2006-10-09, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Polimorfnost STR biljega koristi se u cijelom svijetu kao standardni genetički biljeg za razvoj forenzičkih baza podataka. Prvo se počelo raditi s lokusima sa dinukleotidnim ponavljanjima, no zbog loših karakteristika, poput puno „stutter“ produkata i mikrovarijanata, te težeg očitavanja alela, tražili su se drugi biljezi. Tako su otkriveni STR lokusi s pentanukleotidnim ponavljanjima koji su vrlo polimorfni i heterozigotni, imaju malo „stutter“ produkata i gotovo da nemaju mikrovarijante. Obzirom na svojstva, kao što su reproducibilnost rezultata i pouzdanost analize, komercijalni kompleti su vrlo pogodni za primjenu u slučajevima utvrđivanja očinstva i srodstva, identifikacije posmrtnih ostataka i rješavanja kriminalistickih slučajeva, te stvaranju populacijskih baza podataka. Oni također omogućuju istovremenu amplifikaciju nekoliko lokusa. Cilj ovog rada je bio stvaranje baze podataka lokusa sa pentanukloetidnim ponavljanjima. Analizirani su Penta B, Penta C, Penta D i Penta E lokusi u uzorku hrvatske populacije, a dobiveni rezultati statistički su obrađeni. Uočen je visoki postotak heterozigotnosti, visoki potencijal diskriminacije, te samo jedan mikrovarijant, što čini te lokuse vrlo dobrim za primjenu u forenzici.

Abstract (english)

Polymorphism of STR markers is widely used as the standard genetics marker for the development of forensic databases. Early research used loci with dinucleotide repeats. B ecause of their poor characteristics, such as plenty of stutter products and microvariants, and problems with allel callings, other markers were sought. This lead to the discovery of STR pentanucleotide loci, which are highly polymorphic and heterozygotic, have little stutter products, and almost no microvariants. Considering these characteristics, along with the reproducibility of results and the reliability of analysis, commercial kits are fitting for use in paternity testing, kinship analysis, identification of human remains, criminal cases, and establishing population databases. These kits also allow amplification of several loci at the same time. The purpose of this thesis is to establish Croatian population database for those pentanucleotide loci. Penta B, Penta C, Penta D and Penta E loci were analysed in 100 sample from Croatian population. The results obtained in the analysis were statistically processed. The results of the analysis for Croatian population show a large percentage of heterozygocy, a great potential of discrimination, and only one microvariant. Therefor they are used in Forensic.

• Keywords: STR; pentanukleotidna ponavljanja; PowerPlex 16; Penta BEC; hrvatska populacija
• Keywords (english): STR; pentanucleotide repeats; PowerPlex 16; Penta BEC; Croatian population
• Language: croatian
• URN:NBN: urn:nbn:hr:159:079934
• Study programme: Title: Biotechnology; specializations in: Biochemical-Microbiology; Course: Biochemical-Microbiology; Study programme type: university; Study level: graduate; Academic / professional title: diplomirani inženjer biotehnologije; smjer biokemijsko-mikrobiološki (diplomirani inženjer biotehnologije; smjer biokemijsko-mikrobiološki)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-09-08 09:31:26