Proliferacija neuralnih matičnih stanica u hidrogelovima

undergraduate thesis

• Title (english): Proliferation of neural stem cells in hidrogell
• Author: Mirela Tinjić
• Mentor: Lidija Šver (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); Zagreb
• Defense date and country: 2015-09-09, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Interdisciplinary Technical Sciences

Abstract

Matične stanice su trenutno glavna nada za oporavak i obnovu ozljeđenog tkiva mozga. Vrsta matičnih stanica kojima se daje prednost kod ozljeda mozga su neuralne matične stanice. Proučavanje i shvaćanje interakcija između neuralnih matičnih stanica i biomaterijala može unaprijediti opstanak i prožimanje sa ozljeđenim ili oboljelim predjelima središnjeg živčanog sustava. Zbog svoje biokompatibilnosti, hidrofilnog karaktera i mehaničkih svojstava te strukturalne raznolikosti, hidrogelovi se često koriste za regeneraciju živčanog tkiva. Cilj ovoga rada bio je utvrditi da li se metakrilirana gelan guma (MGG) može koristiti kao dobar hidrogelni nosač neuralnih matičnih stanica, što bi joj omogućilo niz primjena u neuroznanosti. Kako bismo to provjerili, uklopili smo mišje neuralne stanice u hidrogelove gelan gume i izmjerili stupanj umnažanja (proliferacije) stanica dva dana nakon uklapanja. Varijacije u preživljenju i drastično opadanje broja stanica je pokazalo da 1%-tni MGG nije pogodan za neuralne matične stanice.

Abstract (english)

Stem cells are currently the main hope for recovery and reconstruction of injured brain tissue. Type of stem cells preferable in brain injury are neural stem cells. The study and understanding of the interactions between neural stem cells and biomaterials can improve survival and fusion with injured or diseased areas of the central nervous system. Because of its biocompatibility, the hydrophilic character, the mechanical properties and structural diversity, hydrogels are widely used for regeneration of nerve tissue. The aim of this research is to examine whether methacrylated gellan gum (MGG) can be used as a good hydrogel carrier of neural stem cells, which would provide a range of possibilities of applications in neuroscience. To test this, we match mice neural cells in hydrogel gellan gum and measure the degree of multiplication (proliferation) cells five days after switching. Variations in survival and declining cells showed that 1% MGG is not suitable for neural stem cells.

• Keywords: matične stanice; neuralne matične stanice; hidrogel; metakrilirana gelan guma
• Keywords (english): stem cells; nural stem cells; hydrogel; methacrylated gellan gum
• Language: croatian
• URN:NBN: urn:nbn:hr:159:349781
• Study programme: Title: Nutrition; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) nutricionizma (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) nutricionizma)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2015-12-14 12:59:58