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undergraduate thesis
Konstrukcija plazmida pDUK
2012. urn:nbn:hr:159:331178
Stipetić, Tena
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biology and Microbial Genetics

Cite this document

Stipetić, T. (2012). Konstrukcija plazmida pDUK (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:331178

Stipetić, Tena. "Konstrukcija plazmida pDUK." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2012. https://urn.nsk.hr/urn:nbn:hr:159:331178

Stipetić, Tena. "Konstrukcija plazmida pDUK." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2012. https://urn.nsk.hr/urn:nbn:hr:159:331178

Stipetić, T. (2012). 'Konstrukcija plazmida pDUK', Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 23 January 2025, https://urn.nsk.hr/urn:nbn:hr:159:331178

Stipetić T. Konstrukcija plazmida pDUK [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2012 [cited 2025 January 23] Available at: https://urn.nsk.hr/urn:nbn:hr:159:331178

T. Stipetić, "Konstrukcija plazmida pDUK", Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2012. Available at: https://urn.nsk.hr/urn:nbn:hr:159:331178

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Metadata
TitleKonstrukcija plazmida pDUK
Title (english)Construction of plasmid pDUK
AuthorTena Stipetić
MentorIvan-Krešimir Svetec (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Biology and Microbial Genetics)
Zagreb
Defense date and country2012-07-25, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Tehnologija rekombinantne DNA obuhvaća skup metoda koje omogućuju konstrukciju rekombinantnih molekula DNA. Primjena ove tehnologije rezultirala je revolucionarnim otkrićima u biologiji i srodnim znanostima. U ovom radu spomenutim metodama konstruirana su dva nova rekombinantna plazmida, pDUK i pDUK2, ugradnjom fragmenta DNA koji sadrži gen kanMX4 iz plazmida pDMS+ u plazmid pDU. Rezultati restrikcijskih analiza potvrdili su uspješnu konstrukciju oba plazmida. Konstruirani plazmidi sadrže ishodište replikacije za bakteriju Escherichia coli, gene koji omogućuju rezistenciju na antibiotike ampicilin i kanamicin te gen URA3 iz kvasca Dekkera bruxellensis.
Abstract (english)
Recombinant DNA technology includes a group of methods that enable construction of recombinant DNA. The application of this technology has resulted in revolutionary discoveries in biology and related sciences. In this study two new recombinant plasmids were constructed, plasmids pDUK and pDUK2 by inserting DNA fragment that contains gene kanMX4 from plasmid pDMS+ in plasmid pDU. The results of restriction analysis confirmed successful construction of both plasmids. Constructed plasmids contain the origin of replication for bacteria Escherichia coli, genes that provide resistance to antibiotics ampicillin and kanamycin and gene URA3 from yeast Dekkera bruxellensis.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:331178
Study programmeTitle: Biotechnology
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2017-05-16 07:12:06