Primjena ekstrakcije potpomognute mikrovalovima i ultrazvukom u izolaciji polifenolnih spojeva iz korijena i lista maslačka

master's thesis

• Title (english): Application of microwave assisted extraction and ultrasound assisted extraction in isolation of polyphenolic compounds from the dandelion root and leaf
• Author: Matea Mirković
• Mentor: Verica Dragović Uzelac (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Food Engineering); (Laboratory for Technology of Fruits and Vegetables Preservation and Processing); Zagreb
• Defense date and country: 2016-09-27, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Food Technology

Abstract

Cilj ovog rada bio je ispitati utjecaj ekstrakcije potpomognute mikrovalovima (MAE) i ekstrakcije potpomognute ultrazvukom (UAE) na koncentraciju ukupnih fenola u ekstraktima korijena i lista maslačka. Kod obje metode ekstrakcija je provedena s 50 i 70 % vodenim otopinama etanola te vremenu ekstrakcije 5,10 i 15 minuta. Kod MAE su varirane temperature ekstrakcije te je ekstrakcija provedena pri 40°C, 60°C i 80°C, a UAE je provedena pri amplitudi 50% i 100%. Optimalni uvjeti za izolaciju fenolnih spojeva iz korijena maslačka primjenom MAE su temperatura ekstrakcije 60°C, 70% vodena otopina etanola i vrijeme ekstrakcije od 15 minuta, dok je za list maslačka 40°C, 50% vodena otopina etanola i 10 minuta. Optimalni uvjeti za izolaciju fenolnih spojeva iz korijena maslačka primjenom UAE su amplituda 100%, 70% vodena otopina etanola i vrijeme ekstrakcije 10 minuta, dok su za list maslačka amplituda 50%, polarnost 50% i vrijeme ekstrakcije 15 minuta. Također je utvrđeno da se primjenom MAE pri navedenim uvjetima dobivaju veći prinosi ukupnih fenola u usporedbi s UAE.

Abstract (english)

The aim of this study was to investigate the influence of microwave-assisted extraction (MAE) and ultrasonic assisted extraction (UAE) to the concentration of total phenols in extracts of dandelion roots and leaves. In both methods of extraction was carried out with 50 or 70% aqueous ethanol, and the extraction time of 5, 10 and 15 minutes. By MAE extraction temperature were varied, and the extraction was carried out at 40 ° C, 60 ° C and 80 °, and the UAE method was conducted at an amplitude of 50% and 100%. The optimum conditions for the isolation of phenol compounds from root of dandelion using MAE are the extraction temperature of 60 ° C, 70% aqueous ethanol, and the extraction time of 15 minutes, while the conditions for dandelion leaf are 40 ° C, 50% aqueous solution of ethanol in 10 minutes. The optimum conditions for the isolation of phenol compounds from root of dandelion using UAE are 100% amplitude, 70% aqueous ethanol, and the extraction time of 10 minutes, while the conditions for dandelion leaf are 50% amplitude ,50% aqueous ethanol and the extraction time of 15 minutes. It was also found that the application of the MAE methods in these conditions receive higher yields of total phenols in comparison to the UAE.

• Keywords: maslačak; polifenolni spojevi; ekstrakcija; mikrovalovi; ultrazvuk
• Keywords (english): dandelion; polyphenolic compounds; extraction; micro waves; ultrasound
• Language: croatian
• URN:NBN: urn:nbn:hr:159:081415
• Study programme: Title: Nutrition; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra nutricionizma (magistar/magistra nutricionizma)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2017-04-24 08:48:00