Analiza dinamike rasta, promjena metabolizma i proteinskog statusa tijekom dugotrajnog uzgoja sojeva bakterije Lactobacillus plantarum

master's thesis

• Title (english): Analysis of the growth dynamics, metabolism changes and protein status during long-term cultivation of Lactobacillus plantarum bacterial strains
• Author: Marija Milić
• Mentor: Višnja Bačun-Družina (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Biology and Microbial Genetics); Zagreb
• Defense date and country: 2012-11-12, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Mikroorganizami koji rastu u prirodi izloženi su brojnim nepovoljnim fizičkim i kemijskim uvjetima pri čemu su izloženi različitim oblicima stresa. Prilagodba na nove uvjete generacije sojeva ovisi o genomskoj raznolikosti bakterija. U ovom radu nepovoljni uvjeti okoliša u kojima bakterije rastu postignuti su šaržnim uzgojem sedam različitih sojeva Lactobacillus plantarum do produžene stacionarne faze rasta. Tijekom dužeg perioda rasta zabilježen je pad broja živih stanica u hranjivoj podlozi (MRS podloga), dok je u siromašnoj podlozi (MML podloga) zabilježen rast u tri ispitivana soja i tijekom dvadeset i pet dana. Rezultati su pokazali da bakterije mliječne kiseline prilagođavaju svoj metabolizam na uvjete u kojima se nalaze, a tijekom dugotrajnog rasta zabilježen je pad koncentracije mliječne kiseline. S obzirom na različitu starost različitih sojeva uočene su i promjene u sastavu ukupnih proteina, kao i promjena rezistencije na antibiotike. Uočen je izostanak osjetljivosti na nalidiksinsku kiselinu u svih sojeva, dok je na ostale ispitivane antibiotke zabilježena različita osjetljivost.

Abstract (english)

Microorganisms that grow in nature are exposed to a number of adverse physical and chemical conditions wherein are exposed to various forms of stress. Adaptation to new conditions depends on the generation of strains of bacteria genomic diversity. In this work, there were imitated the habitats which bacteria inhabited by harboring seven different Lactobacillus plantarum strains in prolonged stationary growth phase. During the long period of growth the concentration of viable cells in nutrient media (MRS media) decreased, while in poor media (MML media) it was recorded a growth of three studied strains during the twenty-five days. The results showed that lactic acid bacteria adapt their metabolism to the conditions in which they are located, and during long-term growth decreased concentrations of lactic acid. The changes were observed in the composition of total cellular proteins of different ages. The changes of antibiotics resistance cells of different ages were monitored. The lack of sensitivity to nalidixic acid in all strains was noted, while the other tested antibiotics observed different sensitivity dynamics.

• Keywords: Lactobacillus plantarum; produžena stacionarna faza rasta; organske kiseline; ukupni stanični proteini; antibiotička rezistencija
• Keywords (english): lactic acid bacteria; prolonged stationary growth phase species; organic acids; total cellular proteins; antibiotic resistance
• Language: croatian
• URN:NBN: urn:nbn:hr:159:022487
• Study programme: Title: Biotechnology; specializations in: Biochemical-Microbiology; Course: Biochemical-Microbiology; Study programme type: university; Study level: graduate; Academic / professional title: diplomirani inženjer biotehnologije; smjer biokemijsko-mikrobiološki (diplomirani inženjer biotehnologije; smjer biokemijsko-mikrobiološki)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-05-17 09:36:58