Citotoksični učinak PCB 77 i protektivni učinak vitamina E na proliferaciju CHO (Chinese Hamster Ovary)-K1 stanica

master's thesis

• Title (english): Cytotoxic effect of PCB-77 and protective effect of vitamin E on proliferation of CHO-K1 cells
• Author: Adrijana Miletić
• Mentor: Ivana Kmetič (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Chemistry and Biochemistry); (Laboratory for Toxicology); Zagreb
• Defense date and country: 2012-10-11, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Food Technology

Abstract

Poliklorirani bifenili („polychlorinated biphenyls; PCBs“) su teško razgradivi organski zagaĊivaĉi okoliša koji uzrokuju mnoga negativna djelovanja na ţiva bića. Cilj ovog istraţivanja bio je odrediti djelovanje PCB 77 na proliferaciju CHO (Chinese Hamster Ovary)-K1 stanica, te potencijalno protektivno djelovanje vitamina E na PCB-em uzrokovana oštećenja i smanjenu vijabilnost stanica. U tu svrhu stanice su tretirane razliĉitim koncentracijama PCB 77 (10, 25, 50, 75 i 100 µM), a njegov citotoksiĉni uĉinak odreĊen je metodom Trypan Blue tijekom 24, 48 i 72 sata. Inhibitorni uĉinak i toksiĉno djelovanje PCB 77 vidljivo je uz sve primjenjene koncentracije, a taj uĉinak raste s porastom njegove koncentracije i duljinom trajanja tretmana. Primjenom fluorescentne mikroskopije odreĊen je tip staniĉne smrti kojoj podlijeţu stanice tretirane odabranim PCB kongenerom. PCB 77 u većem postotku izaziva apoptozu CHO-K1 stanica nego nekrozu istih. Antioksidans vitamin E u koncentracijama 10 i 50 μg/mL medija za uzgoj djeluje blago protektivno na CHO-K1 stanice tretirane s 50 μM PCB 77, ali taj protektivni uĉinak nije statistiĉki znaĉajan.

Abstract (english)

The polychlorinated biphenyls (PCBs) are persistent organic pollutants that cause many adverse effects on living beings. The objective of this research was to determine effects of PCB-77 on proliferation of CHO (Chinese Hamster Ovary)-K1 cell line and potential protective effect of vitamin E on PCB 77 caused damage and reduced cell viability. CHO-K1 cells were treated with different concentrations of PCB 77 (10, 25, 50, 75 and 100 μM) and its cytotoxic effect was determined by Trypan Blue method after 24, 48 and 72 hours. Inhibitory and toxic effects of PCB 77 were observed for all applied concentrations and these effects increase with the increase of its concentration and duration of treatment. Applying fluorescence microscopy to determinate the type of cell death, it was assessed that PCB 77 induces apoptosis to a greater extent than necrosis. Antioxidant vitamin E in concentration of 10-50 μg/mL of medium exerted protective effect on CHO-K1 cells treated with 50 μM PCB 77, but the extent of protective effect was not statistically significant.

• Keywords: CHO-K1 staniĉna linija; PCB 77; citotoksiĉnost; metoda Trypan Blue; vitamin E
• Keywords (english): CHO-K1 cell line; PCB-77; cytotoxicity; Trypan Blue method; vitamin E
• Language: croatian
• URN:NBN: urn:nbn:hr:159:203350
• Study programme: Title: Food Technology; specializations in: Nutrition; Course: Nutrition; Study programme type: university; Study level: graduate; Academic / professional title: diplomirani inženjer prehrambene tehnologije; smjer nutricionizam (diplomirani inženjer prehrambene tehnologije; smjer nutricionizam)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-05-17 11:25:44