Antifungalni i antipatulinski učinak bakterije Gluconobacter oxydans 1J izolirane s jabuka

master's thesis

• Title (english): Antifungal and antipatulin effect of bacteria Gluconobacter oxydans 1J isolated from apple
• Author: Dragana Mesarek
• Mentor: Ksenija Markov (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for General Microbiology and Food Microbiology); Zagreb
• Defense date and country: 2012, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Svrha ovog rada je bila izolirati s jabuke mikrobnu populaciju i utvrditi moguće inhibitorno djelovanje Gluconobacter oxydans 1J na plavu plijesan, Penicillium expansum 565, najvažnijeg uzročnik kvarenja jabuka u skladištu. Najveća antifungalna aktivnost bakterije je primjećena između 3. i 9. dana, kada nije zabilježen porast kolonija, dok je nakon 12. dana promjer kolonije plijesni manji za 42.3%. Klasičnim mikrobiološkim metodama, API-testom i MALDI- TOF MS analizom, izolirana je i identificirana prirodno prisutna mikroflora jabuka. Iako istraživana bakterija Gluconobacter oxydans 1J nije u potpunosti inhibirala rast plijesni u jabučnom soku, pokazala je visoku razinu učinkovitosti (72-78%). Gluconobacter oxydans 1J djelomično inhibira rast plijesni Penicillium expansum 565 i biosintezu patulina u ovisnosti o vremenu i uvjetima uzgoja.

Abstract (english)

The purpose of this study was to isolate the natural microbial population from apples and investigate the possible inhibition abilities of Gluconobacter oxydans 1J against blue mold Penicillium expansum 565, the most important postharvest decay of apples. The highest antifungal activity of this bacterium was observed between 3 and 9 days with no colony growth, while after 12 days colony diameter was reduced to 42.3 % respectively. Classical microbiological method, API tests and MALDI-TOF MS analysis were used for isolation and identification of the microbial population from apples. Although the investigated bacterium was not completely inhibited mold growth in apple juice, they showed high level of efficiency (72-78%). Gluconobacter oxydans 1J partially inhibit growth mold Penicillium expansum 565 and biosynthesis of patulin, depending on the time and conditions of cultivation.

• Keywords: Gluconobacter oxydans 1J; Penicillium expansum 565; inhibicija; patulin
• Keywords (english): Gluconobacter oxydans 1J; Penicillium expansum 565; inhibition; patulin
• Language: croatian
• URN:NBN: urn:nbn:hr:159:793924
• Study programme: Title: Biotechnology; specializations in: Biochemical Engineering; Course: Biochemical Engineering; Study programme type: university; Study level: graduate; Academic / professional title: diplomirani inženjer biotehnologije; smjer biokemijsko inženjerstvo (diplomirani inženjer biotehnologije; smjer biokemijsko inženjerstvo)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-05-17 11:52:28