Povećani izražaj CCR4 i CXCR3 na limfocitima T u akutnoj RSV-infekciji

master's thesis

• Title (english): Increased expression of CCR4 and CXCR3 lymphocyte T in children with acute RSV-infection
• Author: Marina Prgomet
• Mentor: Višnja Gaurina Srček (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Cell Technology, Application and Biotransformations); Zagreb
• Defense date and country: 2012-07-13, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Kemokini su mali peptidi koji se vežu za specifične receptore te na taj način sudjeluju u usmjerenoj migraciji stanica. Diferencijalna proizvodnja kemokina tijekom određenog tipa imunološke reakcije pospješuje migraciju stanica koje na površini izražavaju specifični kemokinski receptor. Uspješna obrana od virusnih infekcija podrazumijeva razvoj Th1 imunološkog odgovora koji se može povezati s proizvodnjom CXCL9 i CXCL10 te usmjerenom migracijom limfocita T koji izražavaju CXCR3. Međutim, u akutnoj RSV-infekciji djece, uočena je povišena proizvodnja Th2 citokina koji potiču proizvodnju CCL17 i CCL22 i pospješuju migraciju CCR4-pozitivnih limfocita T. U ovom radu utvrdili smo da je u perifernoj krvi djece s akutnom RSV-infekcijom detektiran veći postotak CD4 limfocita T koji na površini izražavaju CCR4 kao i veći postotak CD8 limfocita T koji na površini izražavaju CXCR3 kemokinski receptor, u odnosu na zdravu djecu slične dobi. Dobiveni rezultati potvrđuju da tijekom akutne RSV-infekcije dolazi do razvoja mješovite Th1 i Th2 imunološke reakcije koja se odražava na nivou izražaja kemokina i kemokinskih receptora. Obzirom da se uspješna obrana od virusa temelji predominantno na razvoju Th1 profila, prisustvo Th2 tipa imunološke reakcije u akutnoj RSV-infekciji može objasniti neefikasnu antiviralnu reakciju što doprinosi pojavi teže kliničke slike bolesti.

Abstract (english)

Chemokines are small peptides involved in the recruitment of cells bearing specific chemokine receptors. They are induced by the number of factors, including cytokines that could differently regulate expression of chemokines and their receptors. Viral infections mount Th1 immune reaction that is followed by increased expression of CXCL9 and CXCL10, and efficient local infiltration of CXCR3-positive T lymphocytes at the site of infection. RSV infection also induces Th2 immune response leading to the secretion of CCL17 and CCL22 chemokines. They support migration of CCR4-positive lymphocytes that preferably produce Th2 cytokines. In RSV-infected infants we determined a higher percentage of CCR4-positive CD4 T lymphocytes and higher percentage CXCR3-positive CD8 T lymphocytes than in healthy group. These results confirm previous findings that RSV is able to induce mixed type of immune response at the level of chemokine receptors and could be related to inadequate antiviral immune reaction in infants with severe disease.

• Keywords: kemokini; kemokinski receptori; CCR4; CXCR3; RSV; protočni citometar
• Keywords (english): chemokine; chemokine receptor; RSV; Flow cytometry
• Language: croatian
• URN:NBN: urn:nbn:hr:159:573999
• Study programme: Title: Biotechnology; specializations in: Biochemical-Microbiology; Course: Biochemical-Microbiology; Study programme type: university; Study level: graduate; Academic / professional title: diplomirani inženjer biotehnologije; smjer biokemijsko-mikrobiološki (diplomirani inženjer biotehnologije; smjer biokemijsko-mikrobiološki)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-05-17 12:50:54