Učinak kanolola iz repičinog ulja na kulture stanica

undergraduate thesis

• Title (english): The effect of canolol from rapeseed oil on the cell culture
• Author: Martina Bagović
• Mentor: Kristina Radošević (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Cell Technology, Application and Biotransformations); Zagreb
• Defense date and country: 2016-07-12, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

4-vinil-2,6-dimetoksifenol ili kanolol je polifenolni spoj izoliran iz nerafiniranog repičinog ulja. Prema dosadašnjim studijama kanolol je pokazao izrazita antioksidacijska svojstva, te je dokazano da ima antimutagenu, antikancerogenu i protuupalnu aktivnost. U ovome radu isptivao se antitumorski utjecaj kanolola in vitro, odnosno u kulturi stanica. Kulture stanica se često koriste za ispitivanje biološke aktivnosti prirodnih spojeva, kao i citotoksičnosti istih. Korištene su dvije humane stanične linije, tumorska (HeLa) i stanična linija izvedena iz zdravih stanica (HEK293T). Obje linije su bile izložene različitim koncentracijama kanolola tijekom 72 sata kako bi se potvrdila njegova antitumorska aktivnost i odredile EC50 vrijednosti spoja. Učinak kanolola na HeLa i HEK293T stanice određen je primjenom kolorimetrijske Neutral Red metode, te vizualiziran bojanjem bojom kristal-ljubičasto. Citotoksičan učinak kanolola zapažen je na obje stanične liniije pri koncentracijama >50µM, proporcionalan je povećanju koncentracije kanolola, a izračunate EC50 vrijednosti iznose 268,431 µM za HEK293T stanice te 333,569 µM za HeLa stanice.

Abstract (english)

4-vinyl-2,6-dimethoxyphenol or canolol is a phenolic compound extracted from crude rapeseed oil. The latest studies show that canolol has strong antioxidative effect, as well as antimutagenic, anticancerogenic and anti-inflammatory activity. The goal of this final thesis was to determine antitumor activity of canolol in vitro, by using cell culture. Cell culture is a frequently used method in the examination of biological active natural compounds, as well as their cytotoxicity. Two human cell lines were used, tumor cell line (HeLa) and normal one conducted from healthy cells (HEK293T). Both of the lines were exposed to different concentrations of canolol in the period of 72 hours in order to prove its antitumor activity and determine its EC50 value. The effect of canolol on HeLa and HEK293T cells was determine by application of colorimetric Neutral Red method, and visualized by colouring with crystal-purple colour. Cytotoxic effect of canolol was noted on both cell lines at concentration >50µM, and it’s proportionate to the canolol dose, EC50 values are 268.431 µM for HEK293T cells and 333.569 µM of HeLa cells.

• Keywords: kanolol; repičino ulje; antitumorski aktivnost; kulture stanica
• Keywords (english): canolol; rapeseed oil; antitumor activity; cell culture
• Language: croatian
• URN:NBN: urn:nbn:hr:159:797549
• Study programme: Title: Biotechnology; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2017-04-24 09:30:12