| Abstract | Istraživan je utjecaj sorte i mikroklimatskih uvjeta na sadržaj karotenoida u mandarinama sorti Kawano Wase, Zorica, Chahara, Okitsu, ubranim na pet različitih lokacija u dolini Neretve, u dva termina berbe. Karotenoidni spojevi određivani su primjenom HPLC metode uz UV/Vis PDA detekciju. U ispitivanim sortama identificirano je 9 karotenoida (violaksantin, luteoksantin a i b, lutein, lutein izomer, zeaksantin, β-kriptoksantin, α-karoten, -karoten), a dominantan je β-kriptoksantin (2,47-16,67 mg/kg svjeţe pulpe mandarine) koji je u ukupnoj količini karotenoida zastupljen od 55 do 70%. Najviša koncentracija ukupnih karotenoida određena je u sorti Kawano Wase (8,16-26,13 mg/kg svjeţe pulpe mandarine), a u padajućem nizu slijede Chahara (11,61-23,43 mg/kg), Okitsu i Zorica (8,31-18,67 mg/kg; 4,36-21,22 mg/kg). Područje uzgoja i sortiment značajno utječu na koncentraciju ukupnih ksantofila, dok je sorta mandarine imala značajan utjecaj na koncentraciju β-kriptoksantina (p<0,05). Nadalje, niti jedan od izvora varijacija (sorta mandarine, lokacija uzgoja, termin berbe) nije imao signifikantan utjecaj na koncentracije ukupnih karotena i karotenoida. |
| Abstract (english) | The influence of cultivar and microclimate conditions on the content of carotenoids in mandarin cultivars Kawano Wase, Zorica, Chahara, Okitsu, harvested at five different locations in two terms of harvest, in Neretva valley, was researched. Carotenoid compounds were determined using HPLC with UV/Vis PDA detection. Researched cultivars contained 9 carotenoids (violaxantin, luteoxanitin a and b, lutein, lutein isomer, zeaxsantin, β-cryptoxantin, α-carotene, β-carotene), with β-cryptoxanthin as predominant compound (2,47 to 16,67 mg/kg fresh mandarin orange pulp), present in the total amount of carotenoids from 55 to 70%. The highest concentration of total carotenoids was determined in cultivar Kawano Wase (8,16 to 26,13 mg/kg of fresh mandarin orange pulp), followed by cultivar Chahara (11,61 to 23,43 mg/kg), Okitsu and Zorica (8, 31 to 18,67 mg/kg and 4,36 to 21,22 mg/kg). The microclimate conditions and cultivars significantly affected the concentration of total xanthophylles, while the mandarin cultivar had a significant impact on the concentration of β-cryptoxanthin (p<0,05). Furthermore, none of the sources of variation (mandarin cultivar, breeding locations, date of harvest) had no significant effect on the concentration of total carotenes and carotenoids. |
