Priprava imidazolijevih ionskih tekućina i njihova primjena u biotransformacijama

master's thesis

• Title (english): Preparation of imidazolium ionic liquids and their application in biotransformations
• Author: Ivan Radoš
• Mentor: Jasna Vorkapić-Furač (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Biology and Microbial Genetics); Zagreb
• Defense date and country: 2011-03-25, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Cilj ovog rada bila je sinteza imidazolijevih ionskih tekućina te njihovo ispitivanje kao novih medija za biotransformacije. Ionske tekućine pripravljene su reakcijama kvaternizacije 1,2-dimetilimidazola s 1-brompentanom, 1-bromheksanom i 1-bromheptanom te reakcijama izmjene aniona s litijevim bis[(trifluormetil)sulfonil]imidom. Procesi sinteze provedeni su šaržno, klasičnim postupcima organske sinteze te kontinuirano u mikroreaktorskom sustavu s mikromješačem. Novosintetizirane ionske tekućine karakterizirane su 1H- i 13C-NMR-om. Također, ionske tekućine ispitane su kao novi mediji u reakciji sinteze izoamil-acetata s imobiliziranom lipazom B izoliranom iz kvasca Candida antarctica (Novozym 435), a za usporedbu, reakcija je provedena u komercijalnoj ionskoj tekućini [BMIm][Tf2N].

Abstract (english)

The aim of this study was the synthesis of imidazolium ionic liquids and their testing as new media for biotransformations. Ionic liquids were prepared by quaternization of 1,2-dimethylimidazole with 1-bromopentane, 1-bromohexane and 1-bromoheptane and anion exchange reactions with lithium bis[(trifluoromethyl)sulfonyl]imide. The syntheses were carried out by batch conventional organic synthesis methods, and continuously in a micro reactor system with micro mixer. The novel ionic liquids were characterized by 1H-and 13CNMR spectra. Also, ionic liquids have been examined as new media for the isoamyl acetate synthesis with immobilized lipase B isolated from Candida antarctica (Novozym 435). For the comparison the reaction in commercial ionic liquid [BMIm][Tf2N] was carried out.

• Keywords: imidazolijeve ionske tekućine; izoamil-acetat; mikroreaktorski sustav; mikromiješalo; Novozym 435
• Keywords (english): imidazolium ionic liquids; isoamyl acetate; micro mixer; micro reactor system; Novozym 435
• Language: croatian
• URN:NBN: urn:nbn:hr:159:261004
• Study programme: Title: Bioprocess Engineering; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva (magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-05-30 11:19:57