Rezolucija dvokomponentnog globalnog signala: kriteriji za odabir rezolvirajuće funkcije i raspona signala

master's thesis

• Title (english): Resolution of two component global signal: Criteria for selection of resolving function and signal range
• Author: Darija Ilijaš
• Mentor: Zorana Grabarić (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Chemistry and Biochemistry); (Laboratory for General and Inorganic Chemistry and Electroanalysis); Zagreb
• Defense date and country: 2011-03-17, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Food Technology

Abstract

Metoda omjera spektrofotometrijskih signala korištena je u ovom radu za simultano kvantitativno određivanje bojila Sunset Yellow, Ponceau 4R i Brilliant Black BN u binarnim smjesama. Kako bi se metoda optimirala ispitivan je utjecaj različitih parametara na preciznost dobivenih rezultata. Utvrđeno je da rezolvirajući faktor mora biti definiran na četvrtu decimalu. Optimalan raspon valnih duljina apsorpcijskog spektra za određivanje smjese bojila Sunset Yellow i Ponceau 4R iznosi 450 – 565±5... More nm, a za Ponceau 4R i Brilliant Black 500 – 610±10 nm. Optimale koncentracija rezolvirajućih signala za kvantitativno određivanje bojila u smjesi bile su 15,09 – 63,39 μM za Sunset Yellow i Ponceau 4R i 8,88 – 38,49 μM za Ponceau 4R i Brilliant Black. Koncentracije pojedinih bojila u dvokomponentnoj smjesi u sintetskim uzorcima uspoređene su s koncentracijama dobivenim Vierordtovom metodom. Vrijednosti relativne standardne pogreške metode omjera signala za sve komponente pojedine smjese manje su od vrijednosti relativnih standardnih pogrešaka Vierordtove metode. Less

Abstract (english)

The signal ratio spectrofotometric method was applied in this paper for simultaneous quantitative determination of food dyes Sunset Yellow, Ponceau 4R and Brilliant Black BN in binary mixtures. In order to optimize the signal ratio method, the effect of different parameters on the precision of the obtained results was investigated. It was found that the optimal resolving factor has to be defined with four decimal places thus enabling the best resolution of spectra. The optimal range of... More absorption spectra wavelengths for determination of binary mixtures were for Sunset Yellow and Ponceau 4R from 450 to 565±5 nm and for Ponceau 4R and Brilliant Black from 500 to 610±10 nm. The optimal concentration range of resolving signals for quantitative determination of Sunset Yellow and Ponceau 4R was 15.09-63.39 μM and Ponceau 4R and Brilliant Black 8.88-38.49 μM. The concentration of dyes in synthetic mixtures was determined withVierordt's method, too. Relative standard errors for determination of dyes with signal ratio method for all measurements were lower than when Vierordt's method was used. Less

• Keywords: sintetska bojila; metoda omjera signala; Vierordtova metoda; spektrofotometrija; optimiranje
• Keywords (english): synthetic dyes; signal ratio method; Vierordt's method; spectrophotometry; optimization
• Language: croatian
• URN:NBN: urn:nbn:hr:159:938264
• Study programme: Title: Food Safety Management; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka upravljanja sigurnošću hrane (magistar/magistra inženjer/inženjerka upravljanja sigurnošću hrane)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-05-30 12:11:14