Developing of HPLC-MS/MS method in detection of sulfonamides in eggs

master's thesis

• Title: Razvoj metode HPLC-MS/MS u određivanju sulfonamida u jajima
• Author: Matko Brajnović
• Mentor: Jasna Vorkapić-Furač (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Cell Technology, Application and Biotransformations); Zagreb
• Defense date and country: 2011-11-11, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Sulfonamidi su sintetski bakteriostatici i imaju široku uporabu u veterinarskoj terapiji za liječenje i prevenciju bolesti kod uzgoja govedi, svinja i peradi. Prisutnost sulfonamidnih ostataka u hrani namijenjenoj konzumaciji je opasno radi potencijalnog kancerogenog utjecaja i mogućnosti stvaranja antibakterijske rezistencije. Cilj ovog rada bio je razvoj potvrdne metode koja će uspješno pratiti četiri prethodno odabrana sulfonamida (sulfamerazin, sulfametazin, sulfisoksazol i sulfadoksin) u jajima u skladu s propisanim Pravilnikom o farmakološki djelatnim tvarima i njihovoj klasifikaciji (NN 21/11). Korišteno je 15 uzoraka jaja uzetih s domaćeg tržišta te se pratila koncentracija sulfonamida korištenjem tekućinske kromatografije visoke djelotvornosti s tandem spektrometrometrijom masa. Analize linearnosti, ponovljivosti i iskorištenja ukazale su na koncentracijsko područje na kojem se treba temeljiti daljnji razvoj metode.

Abstract (english)

Sulfonamides are synthetic bacteriostatics and are widely used in veterinary therapy for treating and preventing diseases at farming cows, pigs and poultry. Presence of sulfonamide residues in food is dangerous for potential cancerogenic effect and antibacterial resistance. Aim of this work was to develop a confirmation method that will successfully monitor four selected sulfonamides (sulfamerazine, sulfamethazine, sulfisoxazole, sulfadoxine) in eggs according to Regulation of pharmaceutical active substances and their classification (NN 21/11). Fifteen egg samples from local market have been used to monitor sulfonamides concentration by using high performance liquid chromatography and mass spectrometry. The analysis of linearity, reproducibility and yield indicated the concentration range on which further development of method has to be based.

• Keywords: jaja; spektrometar masa; sulfonamidi; tekućinska kromatografija visoke djelotvornosti
• Keywords (english): eggs; high performance liquid chromatography; mass spectrometry; sulfonamides
• Language: croatian
• URN:NBN: urn:nbn:hr:159:572232
• Study programme: Title: Biotehnologija; smjerovi: Biokemijsko inženjerstvo; Study programme type: sveučilišni; Study level: dodiplomski; Academic / professional title: diplomirani inženjer biotehnologije; smjer biokemijsko inženjerstvo (diplomirani inženjer biotehnologije; smjer biokemijsko inženjerstvo)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Restricted access
• Created on: 2017-05-31 12:35:10