Povezanost jednostrukog nukleotidnog polimorfizma rs1421085 u ljudskom genu FTO s pretilošću žena Zagrebačke županije

master's thesis

• Title (english): Association of single nucleotide polymorphism rs1421085 in human FTO gene with obesity in woman from Zagreb county
• Author: Barbara Smolkovič
• Mentor: Višnja Bačun-Družina (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Biology and Microbial Genetics); Zagreb
• Defense date and country: 2016-07-21, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Pretilost je bolest na koju najčešće utječe više čimbenika te interakcije između okolišnih, socijalnih i genetičkih čimbenika. Svrha ovog istraživanja je ispitati potencijalne razlike u sastavu mikrobioma usne šupljine te potencijalni utjecaj genetičkih faktora na razvoj pretilosti genotipizacijom jednostrukog nukleotidnog polimorfizma rs1421085 gena FTO u pretilih žena i žena normalne težine Zagrebačke županije te analizirati njihove prehrambene navike, stil života i zdravstveno stanje. Ispitivanje povezanosti jednostrukog nukleotidnog polimorfizma rs1421085 gena FTO s pretilošću prvo je takve vrste u sjeverozapadnoj Hrvatskoj i općenito drugo dosad u Hrvatskoj što mu daje dodatni značaj. Dobiveni rezultati pokazuju pozitivnu korelaciju između zastupljenosti visokorizičnog genotipa CC rs1421085 gena FTO i pretilosti žena. Također, pronađene su razlike u sastavu mikrobioma usne šupljine pri čemu je bakterijska vrsta Staphylococcus aureus zastupljenija u pretilih žena, dok su u žena normalne tjelesne težine zastupljenije bakterijske vrste roda Streptococcus (S. infantis, S. mitis, S. oralis i S. salivarius) te bakterijska vrsta Serratia ureilytica. Obrada anketnih upitnika ukazuje da pretile žene u povećanoj mjeri konzumiraju hranu bogatu slatkišima i grickalicama te mesom i mesnim prerađevima i učestalije boluju od povišenog krvnog tlaka u odnosu na kontrolnu skupinu žena. Ovim istraživanjem želio se dobiti uvid u postojanje genetičke podloge za povećani rizik od razvoja pretilosti te detektirati mikroorganizme koji su indikator pretilosti kod žena.

Abstract (english)

Obesity is mainly a multifactorial disease caused by interactions between environmental, social and genetic factors. The aims of this study are to investigate possible differences in composition of the oral microbiota and possible influence of the genetic factors on the obesity risk by genotyping single nucleotide polymorphism rs1421085 in the FTO gene in obese and lean women living in Zagreb county and to analyze their eating habits, lifestyle and health condition. This is the first research in the northwest Croatia and the second research in Croatia till now which examines the association of single nucleotide polymorphism rs1421085 in the FTO gene with woman obesity what gives to this research additional value. The results showed the positive correlation between frequency of the high risk genotype CC rs1421085 of the FTO gene and obesity. Moreover, the differences in the composition of the oral microbiota are shown whereby appearance of bacterial species Staphylococcus aureus is higher in obese group. On the other hand, appearances of bacterial species from genus Streptococcus (S. infantis, S. mitis, S. oralis and S. salivarius) and bacterial species Serratia ureilytica are higher in lean group. The questionnaire analysis showed that obese women prefer candy and snacks rich diet and also consume more meat and meat products and have higher hypertension rate compare to lean
women. The purposes of this study are to get insight of the existence of the genetic basis of increased obesity risk and to detect the microorganisms which indicate to woman obesity.

• Keywords: pretilost; mikrobiom usne šupljine; gen FTO; rs1421085; žene
• Keywords (english): obesity; oral microbiota; FTO gene; rs1421085; women
• Language: croatian
• URN:NBN: urn:nbn:hr:159:904619
• Study programme: Title: Molecular Biotechnology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2017-04-24 10:40:15