Učinak faktora rasta izoliranog iz mozga svinje na proliferaciju CCO stanične linije

undergraduate thesis

• Title (english): Effect of growth factor isolated from porcine brain on CCO cell line proliferation
• Author: Bogdanka Dukić
• Mentor: Višnja Gaurina Srček (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Cell Technology, Application and Biotransformations); Zagreb
• Defense date and country: 2010-07-15, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Proliferacija životinjskih stanica u kulturi pod kontrolom je vanjskih faktora rasta najčešće sadržanih u serumu sisavaca. Faktori rasta sadržani u nekim tkivima poput mozga imaju proliferativan učinak na kulture životinjskih stanica, što je pokazano u prethodnim istraživanjima na primarnim kulturama ovarija, uterusa i kardiomiocita te na BHK21 C13 staničnoj liniji. CCO stanična linija riba sve se više koristi u ekotoksikološkim ispitivanjima i odabrana je u cilju utvrđivanja učinka faktora rasta dobivenog iz mozga svinje na proliferaciju stanica. Na temelju dobivenih rezultata utvrđen je proliferativan učinak sirovog ekstrakta mozga svinje za +14,4% dok je frakcija F3 dobivena nakon pročišćavanja ionsko-izmjenskom kromatografijom pokazala proliferativan učinak od 26,6%.

Abstract (english)

Proliferation of animal cells in culture is uder control of external growth factors, which are usually contained in the mammalian serum. Growth factors contained in some tissues, like brain tissue, have efect on proliferation of cell cultures, which was shown with earlier researches on primary ovarian cultures, uterus and cardiomycetes, and on BHK21 C13 cell line. CCO fish cell line is increasingly used in ecotoxicological researches, and is chosen with the aim of determining the effect of growth factor from porcine brain on cell proliferation. Based on the results it can be concluded that raw extract showed +14,4% proliferative effect, and F3 fraction obtained after ion - exchange purification showed 26,6 % proliferative effect.

• Keywords: CCO stanična linija; faktori rasta; proliferacija; sirovi ekstrakt mozga svinje
• Keywords (english): CCO cell line; growth factors; proliferation; raw porcine brain extract
• Language: croatian
• URN:NBN: urn:nbn:hr:159:757072
• Study programme: Title: Biotechnology; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-06-02 11:44:42