Utjecaj Al2+ i Cd2+ iona na preživljavanje alge Euglena gracilis

undergraduate thesis

• Title (english): Impact of Al2+ i Cd2+ ions on Euglena gracilis survival
• Author: Nina Dobrić
• Mentor: Sunčica Beluhan (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology); Zagreb
• Defense date and country: 2017-07-07, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Stanice algi imaju izvanrednu sposobnost unošenja i nakupljanja teških metala iz vanjskog okoliša. Visoke koncentracije metala, uključujući one esencijalne za rast i metabolizam, toksično djeluju na metabolički sustav algi. Pokretljivost stanica, koncentracije klorofila a i b te karotenoida i nakupljanje prolina, kao ključni toksikološki parametri, proučavani su tijekom 14 dana uzgoja slatkovodnog flagelata Euglena gracilis pri različitim koncentracijama soli teških metala Al2+ i Cd2+ (0.5, 1.0 i 2.0 mM). Stanice E. gracilis bolje podnose izloţenost Al2+ ionima (bolja pokretljivost i bolja sposobnost vršenja fotosinteze). Uočena je značajna osjetljivost na Cd2+ što se očituje i po fotosintetskim pigmentima, pokretljivosti i nakupljanju prolina. Rezultati daju korisne i vaţne informacije o funkcionalnoj vrijednosti E. gracilis u bioremedijacijskim programima.

Abstract (english)

Algal cells have a remarkable ability to uptake and accumulate heavy metals from their external environment. High concentrations of metals, including those essential for growth and metabolism, exert toxic effects on metabolic system of algae. The effects of different concentrations of Al2+ and Cd2+ (0.5, 1.0 and 2.0 mM) on the freshwater flagellate Euglena gracilis were studied upon 14 days of growth using cell mobility, contents of chlorophyll a and b, carotenoids and proline accumulation as end toxicology points. E. gracilis cells showed improved heavy metal tolerance to Al2+ ions (greater mobility and better photosynthetic capacity). Photosynthetic pigments, mobility and proline accumulation were found to be greatly sensitive to Cd2+. These results provide useful and important information about functional value of E. gracilis for bioremediation programs.

• Keywords: Euglena gracilis; fotosintetski pigmenti; pokretljivost; nakupljanje prolina; teški metali
• Keywords (english): Euglena gracilis; Photosynthetic pigments; Motility; Proline accumulation; Heavy metals
• Language: croatian
• URN:NBN: urn:nbn:hr:159:845832
• Study programme: Title: Biotechnology; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2017-06-27 19:36:14