Porast biomase amilolitičke bakterije mliječne kiseline Lactobacillus amylovorus DSM 20531T tijekom uzgoja na maltozi šaržnim postupkom pri suboptimalnoj temepraturi

undergraduate thesis

• Title (english): Increase in biomass of amylolytic lactic acid bacterium Lactobacillus amylovorus DSM 20531T during batch cultivationon maltose at suboptimal temeprature
• Author: Ana Bis
• Mentor: Anita Slavica (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology); Zagreb
• Defense date and country: 2017-07, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Amilolitička bakterija mliječne kiseline Lactobacillus amylovorus DSM 20531T pri suboptimalnoj temperaturi, konstantnoj pH vrijednosti i bez aeracije tijekom šaržnog uzgoja može koristiti maltozu kao izvor ugljika i energije i rasti u odabranim uvjetima. Porast bakterijske biomase može se pratiti određivanjem optičke gustoće izuzete suspenzije kao i prvog razrjeđenja ove suspenzije. Iz dobivenih rezultata procijenjeno je trajanje faza rasta ovog soja kao i specifična brzina rasta. Optička gustoća u skladu je sa podacima dobivenim za koncentraciju suhe tvari bakterijske biomase kao i rezultatima određivanja broja stanica u određenom volumenu suspenzije. Tradicionalne metode za praćenje porasta bakterijske biomase, određivanje faza rasta i procjenu nekih parametara, kao što je specifična brzina rasta bakterijskog soja, pouzdane su i u međusobnom skladu, naročito tijekom eksponencijalne faze rasta radnog mikroorganizma i to u laboratorijskom mjerilu. Na temelju ovih rezultata može se planirati uzgoj ovog potencijalnog industrijskog soja u većem mjerilu uz primjenu ovih metoda.

Abstract (english)

Amylolytic lactic acid bacterium Lactobacillus amylovorus DSM 20531T can use maltose as carbon and energy source during batch cultivation that was carried out at suboptimal temperature, constant pH value and without aeration. Increase in bacterial biomass can be followed by determination of optical density of whithdrawn suspension or its dilution. Obtained results were used in estimation of duration of growth phases as well as for estimation of specific growth rate of L. amylovorus DSM 20531T. Optical density values are in agreement with data obtained by determination of dry weight biomass concentration and number of bacterial cells in defined volume of the suspension. Traditional methods for monitoring of the bacterial biomass increase, estimation of the growth phases duration and other parameters of relevance, e.g. specific growth rate, are reliable and in agreement between each other, especially during exponentialy growth phase of strain when employed at laboratory scale. Based on obtained results it is possible to plan further cultivation experiments of this potential industrial strain at higher scale with implementation of tested methods.

• Keywords: amilolitička bakterija mliječne kiseline; maltoza; šaržni uzgoj; bakterijska biomasa; analitičke metode
• Keywords (english): amylolytic lactic acid bacterium; maltose; batch cultivation; bacterial biomass; analytical methods
• Language: croatian
• URN:NBN: urn:nbn:hr:159:261758
• Study programme: Title: Biotechnology; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2017-07-06 06:28:37