Djelovanje nesteroidnih protuupalnih spojeva na humane stanične linije

undergraduate thesis

• Title (english): The effect of nonsteroidal anti-inflammatory drugs on human cell lines
• Author: Sara Berljavac
• Mentor: Kristina Radošević (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Cell Technology, Application and Biotransformations); Zagreb
• Defense date and country: 2017-07, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Nesteroidni protuupalni lijekovi imaju niz farmakoloških učinka, a u posljednje vrijeme sve je više dokaza u prilog njihove primjene kao antikancerogenih lijekova. Oni mogu umanjiti ili zaustaviti proliferaciju stanica oštećenih slobodnim radikalima, koja može voditi do karcinoma, prvenstveno inhibicijom aktivnosti ciklooksigenaze, enzima ključnog u nizu fizioloških i upalnih procesa. Unatoč njihovoj dokazanoj primjeni kao lijekova protiv karcinoma i nadalje se postavljaju pitanja o njihovoj toksičnosti, sigurnosti i učinkovitosti. U ovom radu ispitan je učinak četiri nesteroidna protuupalna lijeka (ketoprofen, karprofen, fluniksin i 4-metilaminoantipirin monohidrat) primjenom in vitro metode i kultura stanica. Preživljenje stanica HEK293T i HepG2 određeno je primjenom kolorimetrijske MTS metode. Na temelju dobivenih rezultata možemo zaključiti da ispitani lijekovi zaista imaju antiproliferativni učinak na tumorsku staničnu liniju, ali djeluju i na staničnu liniju podrijetlom iz zdravog tkiva. Također, zapaženi inhibitorni učinak ovisi o primjenjenoj dozi te o otapalu u kojem je otopljena djelatna tvar.

Abstract (english)

Non-steroidal anti-inflammatory drugs have a number of pharmacological effects and recently there are evidences of their use as anticancer drugs. They can reduce or stop the proliferation of free radical-depleted cells, which could lead to a cancer, primarily by inhibiting cyclooxygenase activity, a key enzyme in a number of physiological and inflammatory processes. Despite their proven use as a anticancer drug, further questions are raised about their toxicity, safety and efficiency. In this work, four nonsteroidal anti-inflammatory drugs (ketoprofen, carprofen, flunixin and 4-methylaminoantipyrine monohydrate) were tested using the in vitro method and cell culture. The survival of HEK293T and HepG2 cells was determined using a colorimetric MTS method. Based on the obtained results we can conclude that the tested drugs indeed have an antiproliferative effect on the tumor cell line, but also have effect on the cell line from a healthy tissue. The observed inhibitory effect depends on the administered dose and the solvent in which the active substance is dissolved.

• Keywords: antitumorsko djelovanje; humane stanične linije; in vitro citotoksičnost; nesteroidni protuupalni lijekovi
• Keywords (english): antitumor activity; human cell lines; in vitro cytotoxicity; nonsteroidal anti-inflammatory drugs
• Language: croatian
• URN:NBN: urn:nbn:hr:159:149500
• Study programme: Title: Biotechnology; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2017-07-07 12:00:50