Izolacija DNA iz različitih uzoraka tkiva divljih (Felis silvestris Schreber, 1777) i domaćih (Felis catus Linnaeus, 1758) mačaka

undergraduate thesis

• Title (english): DNA extraction from different tissue samples of wild (Felis silvestris Schreber, 1777) and domestic (Felis catus Linnaeus, 1758) cat
• Author: Petra Lindić
• Mentor: Reno Hrašćan (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Biology and Microbial Genetics); Zagreb
• Defense date and country: 2017-07-07, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Izolacija DNA je uklanjanje deoksiribonukleinske kiseline iz svih stanica koje se nalaze u određenom uzorku. Za izolaciju DNA korišten je komplet QIAamp® DNA Mini (Hilden, Njemačka), a sama izolacija izvršena je prema protokolu proizvođača, koji se razlikuje za različite vrste uzoraka. Pomoću tog kompleta izolirana je genomska DNA, tj. DNA koja se ne nalazi samo u jezgrama stanica već i u mitohondrijima. Istraživanje je provedeno na 21 uzorku divljih i 47 uzoraka domaćih mačaka. Uzorci iz kojih se izolirala DNA su: mišićna tkiva divljih mačaka, krv, slina i dlaka domaćih mačaka. Na kraju uspješne izolacije dobiveno je 300 ili 400 μl (ovisno o uzorku iz kojeg se izolira) otopine genomske DNA. Najuspješnije izolacije dobivene su iz krvi i sline, zatim iz mišićnog tkiva, a najneuspješnija izolacija je iz dlake. Od svih vrsta tkiva jedino iz dlake nije niti jednom uspješno izolirana DNA.

Abstract (english)

DNA extraction is the removal of deoxyribonucleic acid from all cells in a particular sample. For DNA extraction, QIAamp® DNA Mini kit (Hilden, Deutschland) is used and extraction was performed according to the manufacturer's protocol, which differs for different types of samples. With this kit genomic DNA is extracted, i.e. DNA that is not only found in the cell nuclei but also in the mitochondria. The study was conducted on 21 wild and 47 domestic cat samples. Samples from which DNA was extracting are: muscle tissue of wild cats, blood, slime and hair of domestic cats. At the end of successful extraction, 300 or 400 μl (depending on the sample from which it is extracted) solution of genomic DNA is obtained. The most successful extractions are obtained from blood and saliva, then from muscle tissue and the most unsuccessful extraction is from the hair. Of all tissue types, only from the hair DNA wasn’t extracted.

• Keywords: izolacija DNA; genomska DNA; divlja mačka; domaća mačka
• Keywords (english): DNA extraction; genomic DNA; wild cat; domestic cat
• Language: croatian
• URN:NBN: urn:nbn:hr:159:544788
• Study programme: Title: Biotechnology; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2017-07-19 08:11:32