Spektrofotometrijsko određivanje polifenola u acetonskim ekstraktima lovora (Laurus nobilis)

undergraduate thesis

• Title (english): Spectrophotometric determination of polyphenols from acetone extracts of laurel (Laurus nobilis)
• Author: Ivona Marjanović
• Mentor: Antonela Ninčević Grassino (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Chemistry and Biochemistry); (Laboratory for Analytical Chemistry); Zagreb
• Defense date and country: 2017-07-17, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Polifenoli su iznimno vaţni zbog svog antioksidativnog djelovanja i pozitivnog učinka na ljudsko zdravlje. Mogu pokazivati i antikorozijska svojstva, a njihova sposobnost adsorpcije na metale ili metalne legure pripisuje se njihovim strukturnim karakteristikama. U ovom radu su pripravljeni acetonski ekstrakti lišća lovora ( = 30 i 70 %) primjenom ultrazvučne ekstrakcije pri vremenu od 3, 6 i 9 min i amplitudi od 60, 80 i 100 %. Maseni udio ukupnih fenola i flavonoida u pripravljenim ekstraktima određen je spektrofotometrijski. Na temelju dobivenih rezultata mjerenja određeni su optimalni uvjeti (70 %-tni aceton, A = 100 % i t = 9 min) ultrazvučne ekstrakcije, koji će se koristiti u daljnjim pripravama ekstrakata pri praćenju njihova antikorozijskog djelovanja.

Abstract (english)

Polyphenols are extremely important because of their antioxidant activity and positive effects on human health. They can show anticorrosion property, and their absorption capability on metals or metal alloys depend on their structural characteristics. In this work, laurel leaves acetone ( = 30 and 70 %) extracts were prepared by ultrassound extraction at time of 3, 6 and 9 min and amplitude of 60, 80 and 100 %. Mass fraction of total phenols and flavonoids in prepared extracts is determined by spectrophotometric method. With regard to obtained results, optimal conditions (70 % acetone, A = 100 % and t = 9 min) of ultrasound extraction were determined and will be used for further preparation of extracts for monitoring of its anticorrosion activity.

• Keywords: antikorozijska i antioksidacijska svojstva; lovor; polifenoli; ultrazvuk
• Keywords (english): anticorrosion and antioxidant activity; laurel; polyphenols; ultrasound
• Language: croatian
• URN:NBN: urn:nbn:hr:159:989995
• Study programme: Title: Biotechnology; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2017-07-20 08:38:14