Primjena proteina Abf2 i MC1 u pripremi kratke kružne DNA za transfekciju stanica u kulturi

master's thesis

• Title (english): Application of Abf2 and MC1 proteins in preparation of short circular DNA for cell transfection
• Author: Ljerka Karleuša
• Mentor: Ivan-Krešimir Svetec (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Biology and Microbial Genetics); Zagreb
• Defense date and country: 2010-07-13, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Jednu od metoda molekulske terapije za modificiranje aktivnosti transkripcijskih faktora čine DNA-mamci (duljine manje od 100 pb). Do sada opisani oblici DNA-mamaca lako se degradiraju endonukleazama. Kružne molekule DNA su rezistentne na taj tip degradacije. Ipak, kratke molekule DNA (kraće od oko 150 pb) ne mogu se cirkularizirati samo uz upotrebu DNA-ligaze U ovom radu opisana je prva uspješna cirkularizacija dvolančane molekule DNA duljine 95 pb uz upotrebu proteina za savijanje DNA (Abf2 i MC1). Isto tako je konstruirana kružna DNA upotrijebljena za transfekciju stanica u kulturi.

Abstract (english)

One of the molecular therapy methods for transcription factor activity modification are DNA-decoys (molecules shorter than 100 bp). All types of DNA-decoys that have been described so far are easily degraded by exonucleases. Circular DNA molecules are, however, resistant to exonuclease activity. Also, it is impossible to create circular DNA from linear dsDNA that is relatively shorter than 150 bp. This is the first published work with described circularization of 95 bp long dsDNA by using Abf2 protein and MC1 protein. The constructed circular DNA was used to transfect cells in cell culture.

• Keywords: DNA-mamci; cirkularizacija DNA; protein Abf2; protein MC1; transfekcija stanica
• Keywords (english): DNA-decoys; DNA circularization; Abf2 protein; MC1 protein; cell culture transfection
• Language: croatian
• URN:NBN: urn:nbn:hr:159:209060
• Study programme: Title: Biotechnology; specializations in: Biochemical-Microbiology; Course: Biochemical-Microbiology; Study programme type: university; Study level: graduate; Academic / professional title: diplomirani inženjer biotehnologije; smjer biokemijsko-mikrobiološki (diplomirani inženjer biotehnologije; smjer biokemijsko-mikrobiološki)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-08-24 07:44:21