Praćenje bioprocesa uklanjanja teških metala u horizontalnom rotirajućem cijevnom bioreaktoru tankoslojnom kromatografijom

master's thesis

• Title (english): Monitoring bioprocess of heavy metal ions removal in horizontal rotating tubular bioreactor by thin-layer chromatography
• Author: Marina Zelenić
• Mentor: Tonči Rezić (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology); Zagreb
• Defense date and country: 2010-03-26, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

U ovom istraţivanju praćeno je uklanjanje metalnih iona (Co2+, Cr6+ i Mn2+) u procesu biosorpcije u horizontalnom rotirajućem cijevnom bioreaktoru (HRCB). HRCB je konstruiran kao kombinacija bioreaktora s tankim slojem i biodisk reaktora te kao takav posjeduje karakteristike oba reaktora. Proces biosorpcije pratio se tankoslojnom kromatografijom (TK). Prije same analize uzoraka otpadne vode bilo je potrebno odrediti optimalni kromatografski sustav za razdvajanje iona metala. Na temelju dobivenih kromatograma i izračunatih faktora zaostajanja (RF vrijednosti), kao i praćenjem vremena razvijanja kromatografskih pločica odabran je najpovoljniji kromatografski sustav za odjeljivanje i identifikaciju analiziranih metalnih iona koji uključuje celulozne pločice kao nepokretnu fazu i razvijač sastava n-propanol:HCl:acetonitril=6:10:4 [v/v] kao pokretnu fazu kromatografskog sustava. Rezultati istraţivanja su pokazali da se ioni Co2+ i Cr6+ mogu odrediti semikvantitativno usporedbom širine mrlje i intenziteta obojenja sa standardom, dok se ioni Mn2+ mogu odrediti samo kvalitativno s obzirom na boju mrlje. Usporedbom koncentracija Co2+ i Cr6+ određenih pomoću TK pri različitim kombinacijama procesnih parametara vidljivo je da brzina protoka ima veći utjecaj na bioproces uklanjanja teških metala u HRCB-u od brzine vrtnje bioreaktora. Smanjenje koncentracije iona metala uzduţ bioreaktora ukazuje na mogućnost primjene HRCB-a u obradi otpadnih voda opterećenih teškim metalima.

Abstract (english)

In this research, removal of metal ions (Co2+, Cr6+ and Mn2+) by biosorption process in horizontal rotating tubular bioreactor (HRTB), was monitored. HRTB is designed as a combination of thin-layer and biodisc reactor and therefore has characteristics of both reactors. Biosorption process was monitored by thin-layer chromatography (TLC). Before analysis of waste water samples, it was needed to determine the optimal chromatographic system for separating metal ions. Based on the obtained chromatograms, calculated retention factors (RF values) and plate development time, best chromatographic system for separation and identification of analyzed metal ions was chosen. This system included cellulose plates as stationary phase, and the solvent mixture composed of n-propanol:HCl:acetonitrile=6:10:4 [v/v] as mobile phase. The research results showed that the Co2+ and Cr6+ ions can be determined semiquantitatively comparing the size and colour intensity of spots with standard. Based on their colour the Mn2+ ions can be only qualitatively determined. Comparison of the concentrations of Co2 + and Cr6 + obtained by TLC at different combinations of process parameters showed that inflow rate has a greater impact on bioprocess removal of heavy metals in HRTB than rotation speed of bioreactor. Decrease of metal ions concentration along the bioreactor length indicates the potential of applying HRTB in the processing of waste water loaded with heavy metals.

• Keywords: ioni teških metala; biosorpcija; tankoslojna kromatografija (TK); biološka obrada otpadnih voda; horizontalni rotirajući cijevni bioreaktor (HRCB)
• Keywords (english): heavy metal ions; biosorption; thin-layer chromatography (TLC); biological waste water treatement; horizontal rotating tubular bioreactor (HRTB)
• Language: croatian
• URN:NBN: urn:nbn:hr:159:063914
• Study programme: Title: Biotechnology; specializations in: Biochemical Engineering; Course: Biochemical Engineering; Study programme type: university; Study level: graduate; Academic / professional title: diplomirani inženjer biotehnologije; smjer biokemijsko inženjerstvo (diplomirani inženjer biotehnologije; smjer biokemijsko inženjerstvo)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-08-25 12:21:38