Utjecaj gnojidbe na biološki aktivne spojeve batata

master's thesis

• Title (english): The influence of fertilization on biolologically active compounds in sweet potato (Ipomoea batatas L.)
• Author: Dubravko Belko
• Mentor: Ivana Radojčić Redovniković (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Cell Technology, Application and Biotransformations); Zagreb
• Defense date and country: 2010-10-07, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Batat je visoko energetska kultura bogata prehrambenim vlaknima, mineralima, vitaminima i antioksidansima, kao što su polifenoli, tokoferoli i β-karoten. Korijen batata rabi se kao hrana i kao industrijski izvor škroba, dočim su listovi u velikoj mjeri zanemareni, izuzev uporabe kao stočne hrane. U radu je ispitan utjecaj mineralnih gnojiva s različitim omjerom dušika i kalija (1:2,5 i 1:5) te dodatak Zeolita i Fitolife-a na udjel ukupnih polifenola i antioksidacijski kapacitet u polifenolnim ekstraktima korijena i listova kultivara batata sorte Bat 1. U uzorcima lista također je određen sastav i udjel flavonoida pomoću HPLC-a. U listu je utvrđen značajno veći udjel ukupnih polifenola i antioksidacijski kapacitet nego u korijenu. Mineralna gnojiva i dodaci utječu na povećanje udjela polifenola u korijenu, dok za list značajna razlika nije utvrđena. Od flavonoida u listovima su identificirani miricetin, kvercetin, apigenin i kamferol. U oba pokusa utvrđen je značajan porast ukupnih flavonoida.

Abstract (english)

Sweet potato is a highly energetic culture rich in dietary fiber, minerals, vitamins and antioxidants such as polyphenols, tocopherols and β-carotene. The roots of sweet potato are used as food and as an industrial source of starch, whereas leaves are largely ignored, except for use as animal feed. The object of this study was to determine the total polyphenolic content and antioxidant activity of root and leaf samples of sweet potato cultivar grown with different fertilization (nitrogen-potassium ratios 1:2.5 and 1:5) and Zeolite and Fitolife addition, and also to determine the flavonoid content of leaf samples by HPLC. The results showed that sweet potato leaves contained more polyphenols than the roots and also have higher antioxidant capacity. Mineral fertilizers and additives affect the increase of polyphenols in the root, but not in the leaves. Four flavonoids were identified: myricetin, quercetin, kaempferol and apigenin. There was a significant increase of total flavonoids in both trials.

• Keywords: batat; flavonoidi; ORAC metoda; polifenolni spojevi
• Keywords (english): flavonoids; ORAC assay; polyphenols; sweet potato
• Language: croatian
• URN:NBN: urn:nbn:hr:159:554850
• Study programme: Title: Biotechnology; specializations in: Biochemical Engineering; Course: Biochemical Engineering; Study programme type: university; Study level: graduate; Academic / professional title: diplomirani inženjer biotehnologije; smjer biokemijsko inženjerstvo (diplomirani inženjer biotehnologije; smjer biokemijsko inženjerstvo)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-08-28 08:18:59